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Recombinant Human IFN-alpha 2 Protein

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SDS-Page: Recombinant Human Interferon alpha 2b Protein [NBP2-34978]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Human IFN-alpha 2 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 166 amino acids corresponding to IFN-alpha 2 Source: E. coli

Uniprot ID: P01563

Amino Acid Sequence: MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE

Details of Functionality
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 x 10^8 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
IFNA2
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of IFN-alpha 2 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human IFN-alpha 2 Protein

  • alpha-2a interferon
  • Ifa2
  • IFNA
  • IFNA2
  • IFNA2a
  • IFNA2b
  • IFNA2c
  • IFNAA
  • IFNalpha 2
  • IFN-alpha 2
  • IFN-alpha-2
  • IFN-alphaA
  • INFA2
  • interferon alpha 2b
  • interferon alpha A
  • interferon alpha-2
  • Interferon alpha-A
  • interferon, alpha 2
  • LeIF A
  • MGC125764
  • MGC125765

Background

IFN-alphas are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol IFNA2
Uniprot