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ID4 Antibody (PCRP-ID4-1A1) - Azide and BSA Free

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Analysis of Protein Array containing more than 19,000 full-length human proteins using ID4 Mouse Monoclonal Antibody (PCRP-ID4-1A1).
Analysis of Protein Array containing more than 19,000 full-length human proteins using ID4 Mouse Monoclonal Antibody (PCRP-ID4-1A1).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
PCRP-ID4-1A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20700AF350
Alexa Fluor 405
NBP3-20700AF405
Alexa Fluor 488
NBP3-20700AF488
Alexa Fluor 532
NBP3-20700AF532
Alexa Fluor 594
NBP3-20700AF594
Alexa Fluor 647
NBP3-20700AF647
Alexa Fluor 700
NBP3-20700AF700
Alexa Fluor 750
NBP3-20700AF750
Allophycocyanin
NBP3-20700APC
Biotin
NBP3-20700B
CoraFluor 1
NBP3-20700CL1
DyLight 350
NBP3-20700UV
DyLight 405
NBP3-20700V
DyLight 488
NBP3-20700G
DyLight 550
NBP3-20700R
DyLight 594
NBP3-20700DL594
DyLight 650
NBP3-20700C
DyLight 680
NBP3-20700FR
DyLight 755
NBP3-20700IR
FITC
NBP3-20700F
HRP
NBP3-20700H
Janelia Fluor 525
NBP3-20700JF525
Janelia Fluor 549
NBP3-20700JF549
Janelia Fluor 585
NBP3-20700JF585
Janelia Fluor 635
NBP3-20700JF635
Janelia Fluor 646
NBP3-20700JF646
Janelia Fluor 669
NBP3-20700JF669
PE
NBP3-20700PE
PerCP
NBP3-20700PCP
Unconjugated
NBP3-20624-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20624-100ug
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ID4 Antibody (PCRP-ID4-1A1) - Azide and BSA Free Summary

Description
Positive Controls: HeLa or U87 cells.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Immunogen
Recombinant fragment (around aa 62-112) of human ID4 protein
Localization
Nucleus.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ID4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation 1-2 ug per 100-500 ug of protein or 1ml of lysate
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for ID4 Antibody (PCRP-ID4-1A1) - Azide and BSA Free

  • bHLHb27BHLHB27
  • Class B basic helix-loop-helix protein 27
  • DNA-binding protein inhibitor ID-4
  • IDB4
  • Inhibitor of DNA binding 4
  • inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

Background

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ID4 Antibody
(PCRP-ID4-1A1) - Azid ...
NBP3-20700
ID4 Antibody (PCRP-ID4-1A1) - Azide and BSA Free
Species: Hu
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ID4