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ID4 Antibody (OTI2G10)

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Western Blot: ID4 Antibody (2G10) [NBP2-46017] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID4 .
Western Blot: ID4 Antibody (2G10) [NBP2-46017] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID4 .

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
OTI2G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70987AF350
Alexa Fluor 405
NBP2-70987AF405
Alexa Fluor 488
NBP2-70987AF488
Alexa Fluor 532
NBP2-70987AF532
Alexa Fluor 594
NBP2-70987AF594
Alexa Fluor 647
NBP2-70987AF647
Alexa Fluor 700
NBP2-70987AF700
Alexa Fluor 750
NBP2-70987AF750
Allophycocyanin
NBP2-70987APC
Biotin
NBP2-70987B
CoraFluor 1
NBP2-70987CL1
DyLight 350
NBP2-70987UV
DyLight 405
NBP2-70987V
DyLight 488
NBP2-70987G
DyLight 550
NBP2-70987R
DyLight 594
NBP2-70987DL594
DyLight 650
NBP2-70987C
DyLight 680
NBP2-70987FR
DyLight 755
NBP2-70987IR
FITC
NBP2-70987F
HRP
NBP2-70987H
Janelia Fluor 525
NBP2-70987JF525
Janelia Fluor 585
NBP2-70987JF585
Janelia Fluor 635
NBP2-70987JF635
Janelia Fluor 669
NBP2-70987JF669
PE
NBP2-70987PE
PerCP
NBP2-70987PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70987
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ID4 Antibody (OTI2G10) Summary

Immunogen
Full length human recombinant protein of human ID4 (NP_001537) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ID4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
Theoretical MW
16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ID4 Antibody (OTI2G10)

  • bHLHb27BHLHB27
  • Class B basic helix-loop-helix protein 27
  • DNA-binding protein inhibitor ID-4
  • IDB4
  • Inhibitor of DNA binding 4
  • inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

Background

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ID4