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Human Total beta-Catenin DuoSet IC ELISA, 2 Plate

 (9 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
DYC1329-5
Biotin
DYC1329E
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Human Total beta-Catenin DuoSet IC ELISA, 2 Plate Summary

Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
CTNNB1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DYC1329-2 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total beta-Catenin DuoSet IC ELISA, 2 Plate

  • bCatenin
  • beta 1 (88kD)
  • beta-Catenin
  • catenin (cadherin-associated protein), beta 1, 88kDa
  • catenin beta-1
  • CTNNB
  • CTNNB1
  • DKFZp686D02253
  • FLJ25606
  • FLJ37923

Background

beta-Catenin, also known as CTNNB1, is a cadherin-associated protein that is involved in the regulation of cell adhesion. This multifunctional protein is also implicated in the canonical Wnt signal transduction pathway, acting as a transcriptional co-activator. Nuclear beta-Catenin activates the TCF/LEF family of transcription factors, inducing the transcription of pro-mitotic genes.

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Human Total
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DYC1329-2
Human Total beta-Catenin DuoSet IC ELISA, 2 Plate
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Publications for beta-Catenin (DYC1329-2)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ELISA Capture.


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ELISA Capture
(1)
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Human
(8)
Mouse
(1)
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Showing Publications 1 - 9 of 9.
Publications using DYC1329-2 Applications Species
H Shailesh, KS Siveen, S Sif Protein arginine methyltransferase 5 (PRMT5) activates WNT/&beta-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3 Journal of Cellular and Molecular Medicine, 2021-01-18;0(0):. 2021-01-18 [PMID: 33462997] (Human) Human
BH Clough, S Zeitouni, U Krause, CD Chaput, LM Cross, AK Gaharwar, CA Gregory Rapid Osteogenic Enhancement of Stem Cells in Human Bone Marrow Using a Glycogen-Synthease-Kinase-3-Beta Inhibitor Improves Osteogenic Efficacy In Vitro and In Vivo Stem Cells Transl Med, 2018-02-05;0(0):. 2018-02-05 [PMID: 29405665] (ELISA Capture, Human) ELISA Capture Human
L Akbarzadeh, T Moini Zanj, M Sabetkasae Comparison of Anticancer Effects of Carbamazepine and Valproic Acid Iran Red Crescent Med J, 2016-08-04;18(10):e37230. 2016-08-04 [PMID: 28184324] (Human) Human
Umbreit C, Flanjak J, Weiss C, Erben P, Aderhold C, Faber A, Stern-Straeter J, Hoermann K, Schultz J Incomplete epithelial-mesenchymal transition in p16-positive squamous cell carcinoma cells correlates with beta-catenin expression. Anticancer Res, 2014-12-01;34(12):7061-9. 2014-12-01 [PMID: 25503133] (Human) Human
Takei Y, Takigahira M, Mihara K, Tarumi Y, Yanagihara K The Metastasis-Associated microRNA miR-516a-3p Is a Novel Therapeutic Target for Inhibiting Peritoneal Dissemination of Human Scirrhous Gastric Cancer. Cancer Res., 2010-12-17;71(4):1442-1453. 2010-12-17 [PMID: 21169410] (Human) Human
Gunn WG, Krause U, Lee N, Gregory CA Pharmaceutical inhibition of glycogen synthetase kinase-3beta reduces multiple myeloma-induced bone disease in a novel murine plasmacytoma xenograft model. Blood, 2010-12-01;117(5):1641-51. 2010-12-01 [PMID: 21123822] (Mouse) Mouse
Matushansky I, Hernando E, Socci ND, Mills JE, Matos TA, Edgar MA, Singer S, Maki RG, Cordon-Cardo C Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway. J. Clin. Invest., 2007-11-01;117(11):3248-57. 2007-11-01 [PMID: 17948129] (Human) Human
Bhattacharyya S, Borthakur A, Dudeja PK, Tobacman JK Carrageenan reduces bone morphogenetic protein-4 (BMP4) and activates the Wnt/beta-catenin pathway in normal human colonocytes. Dig. Dis. Sci., 2007-04-12;52(10):2766-74. 2007-04-12 [PMID: 17429723] (Human) Human
Dolled-Filhart M, McCabe A, Giltnane J, Cregger M, Camp RL, Rimm DL Quantitative in situ analysis of beta-catenin expression in breast cancer shows decreased expression is associated with poor outcome. Cancer Res., 2006-05-15;66(10):5487-94. 2006-05-15 [PMID: 16707478] (Human) Human

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Additional beta-Catenin Products

Blogs on beta-Catenin.

Check out the latest blog posts on beta-Catenin.

  Read full blog post.
Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.
The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.
Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.
Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.
CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.
Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human beta -catenin (1 ng) was included. A specific band for beta-Catenin was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Bioinformatics

Gene Symbol CTNNB1