![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77682] - HSP90-1A antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77682-img0005.jpg "Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77682] - HSP90-1A antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.14 mg/ml |
| Immunogen | A synthetic peptide made to an N-terminal portion of the human Hsp90A protein (between residues 1-70) [UniProt P07900] |
| Localization | Cytoplasm. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HSP90AA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.14 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for HSP90 alpha Antibody (NBP1-77682)Find related products by research area.
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Hsp90A - helping to fold a wide variety of signal transduction proteins Heat-shock protein 90 (Hsp90) is a conserved ATP-dependent chaperone with diverse cellular functions. Hsp90 is ubiquitously expressed but is further induced upon cellular stress such as heat-shock or nutrient deprivation. Hsp90 is essential for the... Read full blog post. |
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![Immunohistochemistry: HSP90 alpha Antibody [NBP1-77682] - Analysis of Hsp901a in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-NBP1-77682-img0006.jpg "Immunohistochemistry: HSP90 alpha Antibody [NBP1-77682] - Analysis of Hsp901a in human kidney carcinoma using DAB with hematoxylin counterstain.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")