Home » HSP10/EPF » HSP10/EPF Antibodies » HSP10/EPF Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

HSP10/EPF Antibody [Unconjugated]

 
 Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Western blot shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
Simple Western lane view shows lysates of DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP10/EPF at approximately 9 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse HSP10/EPF ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2584
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2584-SP
100% Guarantee on every product

HSP10/EPF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse HSP10/EPF
Ala2-Asp102
Accession # Q64433
Specificity
Detects mouse HSP10/EPF in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Hspb2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP10/EPF Antibody [Unconjugated]

  • 10 kDa chaperonin
  • 10 kDa heat shock protein, mitochondrial
  • Chaperonin 10 Homolog
  • Chaperonin 10
  • CPN10HSP10
  • Early-pregnancy factor
  • EPF
  • GroES
  • heat shock 10kD protein 1 (chaperonin 10)
  • heat shock 10kDa protein 1 (chaperonin 10)
  • HSP10
  • HSPE1

Background

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Protein 10 (HSP10), also known as chaperonin 10 (Cpn10), HSPE1, and Early Pregnancy Factor (EPF), is a 10 kDa heat shock protein that functions within the cell as a molecular chaperone and is associated with HSP60 in the mitochondria. Early pregnancy factor (EPF) has been identified as an extracellular homologue of HSP10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HSP60 Antibody -
BSA Free
NBP1-77397
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
(4 Reviews)
(6 Publications)
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
TST Antibody
NBP1-87148
Immunohistochemistry-Paraffin TST Antibody
Immunohistochemistry-Paraffin TST Antibody
Immunohistochemistry-Paraffin TST Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
DNAJB6 Antibody
(2C11-C1)
H00010049-M01
ELISA DNAJB6 Antibody (2C11-C1)
Western Blot DNAJB6 Antibody (2C11-C1)
Western Blot DNAJB6 Antibody (2C11-C1)
(9 Publications)
Species: Hu
Applications: ELISA, ICC/IF (-), IHC, WB
DNAJA2 Antibody
(OTI3A10)
NBP2-02996
Flow Cytometry DNAJA2 Antibody (OTI3A10)
Western Blot DNAJA2 Antibody (OTI3A10)
Immunohistochemistry-Paraffin DNAJA2 Antibody (OTI3A10)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
ME1 Antibody
NBP1-32398
Western Blot ME1 Antibody
Immunocytochemistry/ Immunofluorescence ME1 Antibody
Western Blot ME1 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
ME2 Antibody
NBP1-89559
Immunohistochemistry-Paraffin ME2 Antibody
Immunohistochemistry-Paraffin ME2 Antibody
Immunohistochemistry-Paraffin ME2 Antibody
(4 Publications)
Species: Hu
Applications: IHC, IHC-P, WB
ME3 Antibody -
Azide and BSA Free
NBP3-05078
Immunocytochemistry/ Immunofluorescence ME3 Antibody - Azide and BSA Free
Western Blot ME3 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin ME3 Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
HspA4 Antibody
NBP1-81696
Western Blot HspA4 Antibody
Immunohistochemistry-Paraffin HspA4 Antibody
Western Blot HspA4 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(803 Publications)
Species: Hu
Applications: BA
CLPP Antibody
NBP1-89557
Immunohistochemistry-Paraffin CLPP Antibody
Immunohistochemistry-Paraffin CLPP Antibody
Immunohistochemistry-Paraffin CLPP Antibody
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
Dihydrofolate
Reductase/DHFR Antibo ...
H00001719-M01
Western Blot Dihydrofolate Reductase/DHFR Antibody (2B10)
Western Blot Dihydrofolate Reductase/DHFR Antibody (2B10)
Western Blot Dihydrofolate Reductase/DHFR Antibody (2B10)
(12 Publications)
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, S-ELISA, WB
Steroid sulfatase
Antibody
NBP1-90095
Immunohistochemistry-Paraffin Steroid sulfatase Antibody
Immunohistochemistry-Paraffin Steroid sulfatase Antibody
Immunohistochemistry-Paraffin Steroid sulfatase Antibody
(3 Publications)
Species: Hu
Applications: IHC, IHC-P
CS Citrate
Synthase Antibody (17
NBP2-43648
Western Blot CS Citrate Synthase Antibody (1761)
Western Blot CS Citrate Synthase Antibody (1761)
Western Blot CS Citrate Synthase Antibody (1761)
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
HSP90 alpha
Antibody
NB120-2928
Immunohistochemistry HSP90 alpha Antibody
Immunohistochemistry HSP90 alpha Antibody
Immunohistochemistry-Paraffin HSP90 alpha Antibody
(11 Publications)
Species: Hu, Mu, Pm, Rb, Rt, Sh
Applications: ICC/IF, IHC, IHC-P, IP, WB
CPN1 Antibody
NBP1-57960
Western Blot CPN1 Antibody
Immunohistochemistry-Paraffin CPN1 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
MBP Antibody (12)
NB600-717
Immunohistochemistry-Frozen MBP Antibody (12)
Immunohistochemistry-Frozen MBP Antibody (12)
Immunocytochemistry/ Immunofluorescence MBP Antibody (12)
(3 Reviews)
(41 Publications)
Species: Bv, Ca, Ch, Fe, Gp, Hu, Ma, Mu, Po, Rb, Rt, Sh
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
Glutamine
Synthetase Antibody
NB110-41404
Immunocytochemistry/ Immunofluorescence Glutamine Synthetase Antibody
Western Blot Glutamine Synthetase Antibody
Immunohistochemistry Glutamine Synthetase Antibody
(1 Review)
(10 Publications)
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
HSP10/EPF Antibody
[Unconjugated]
AF2584
HSP10/EPF Antibody [Unconjugated]
Species: Mu
Applications: WB, Simple Western

Publications for HSP10/EPF Antibody (AF2584) (0)

There are no publications for HSP10/EPF Antibody (AF2584).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSP10/EPF Antibody (AF2584) (0)

There are no reviews for HSP10/EPF Antibody (AF2584). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSP10/EPF Antibody (AF2584) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HSP10/EPF Products

Blogs on HSP10/EPF

There are no specific blogs for HSP10/EPF, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HSP10/EPF Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol Hspb2
Uniprot