Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse lactating tissues. PVDF membrane was probed with 1 µg/mL of Chicken Anti-Human/Mouse HMGB1/HMG‑1 Antigen Affinity-purified ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HMGB1/HMG-1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Chicken ...read more
Mouse myeloma cell line NS0-derived recombinant human HMGB1/HMG‑1 Met1-Glu215 Accession # P09429.3
Specificity
Detects human and mouse HMGB1/HMG‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) HMGB3 is observed, and less than 1% cross-reactivity with rhHMGA1, rhHMGA2, and rhHMGA1b is observed.
Source
N/A
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
HMGB1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for HMGB1/HMG-1 Antibody [Unconjugated]
Amphoterin
high mobility group box 1
High mobility group protein 1
high mobility group protein B1
high-mobility group (nonhistone chromosomal) protein 1
high-mobility group box 1
HMG1
HMG-1
HMG1DKFZp686A04236
HMG3
HMGB1
SBP-1
Sulfoglucuronyl carbohydrate binding protein
Background
Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1-3). Human HMGB1 is expressed as a 25-30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu (4, 5). Residues 27-43 and 178-184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues (6). HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.
Lotze, M.T. and K.J. Tracey (2005) Nat. Rev. Immunol. 5:331.
Yang, H. et al. (2005) J. Leukoc. Biol. 78:1.
Dumitriu, I.E. et al. (2005) Trends Immunol. 26:381.
Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
Bonaldi, T. et al. (2003) EMBO J. 22:5551.
Muller, S. et al. (2001) EMBO J. 20:4337.
Bustin, M. (1999) Mol. Cell. Biol. 19:5237.
Wang, H. et al. (1999) Science. 285:248.
Dimitriu, I.E. et al. (2005) J. Immunol. 174:7506.
Najima, Y. et al. (2005) J. Biol. Chem. 280:27523.
Gardella, S. et al. (2002) EMBO Rep. 3:995.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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