HMGB1/HMG-1 Antibody (1F3)

HMGB1 (High-mobility group protein box 1) is part of the high-mobility group proteins which were originally named this way since they are abundant, relatively low molecular weight proteins which run quickly on SDS-PAGE gels. The "box" in the name refers to a compact domain involved in DNA binding and protein-protein interactions. The HMGB1 molecule has two of these HMG domains. The protein is also called amphoterin, this name being derived from the presence of two highly charged regions in the molecule, a relatively neutrally charged N-terminus and a very negatively charged C-terminus. In fact, the molecule is very unusually charged throughout the human sequence consisting of 16.7% Glutamic acid, 9.3% Aspartic acid, 20% Lysine and 9.3% Arginine. HMGB1 can bind Toll like receptor 4 (TLR4) and the Receptor for Advanced Glycation End products (RAGE). TLRs are components of the innate immune system, first recognized as a family of receptors which recognize Pathogen Associated Molecular Pattern molecules (PAMPs). PAMPs are common components of bacteria and when TLRs bind these, a strong inflammatory response is activated. More recently it has been recognized that TLRs can also be activated by Damage Associated Molecular Pattern molecules (DAMPs), which are endogenous substances released from damaged and diseased cells which also bind to TLR family receptors and also activate inflammation. HMGB1 is such a DAMP, binding to TLR4, and much evidence suggests that HMGB1 is a strong activator of inflammation. Interestingly, HMGB1 is released by necrotic cells but not by apoptotic cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, In Vivo.

Showing Publications 1 - 2 of 2.

Publications using NBP2-25148	Applications	Species
Tonner H, Hunn S, Auler N et al. A Monoclonal Anti-HMGB1 Antibody Attenuates Neurodegeneration in an Experimental Animal Model of Glaucoma International journal of molecular sciences 2022-04-07 [PMID: 35456925] (In Vivo, Rat)	In Vivo	Rat
Nestor J, Arinuma Y, Huerta TS et al. Lupus antibodies induce behavioral changes mediated by microglia and blocked by ACE inhibitors. J. Exp. Med. 2018-09-05 [PMID: 30185634] (ICC/IF, Mouse)	ICC/IF	Mouse

Array NBP2-25148

• HMGB1/HMG-1 Antibodies
• HMGB1/HMG-1 Antibody Pair
• HMGB1/HMG-1 ELISA Kits
• HMGB1/HMG-1 Lysate
• HMGB1/HMG-1 Proteins
• HMGB1/HMG-1 Proteins and Enzyme