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HLA ABC Antibody (YTH862.2) [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP
Clone
YTH862.2
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65411AF350
Alexa Fluor 405
NB100-65411AF405
Alexa Fluor 488
NB100-65411AF488
Alexa Fluor 532
NB100-65411AF532
Alexa Fluor 594
NB100-65411AF594
Alexa Fluor 647
NB100-65411AF647
Alexa Fluor 700
NB100-65411AF700
Alexa Fluor 750
NB100-65411AF750
Allophycocyanin
NB100-65411APC
Allophycocyanin/Cy7
NB100-65411APCCY7
Biotin
NB100-65411B
DyLight 350
NB100-65411UV
DyLight 405
NB100-65411V
DyLight 488
NB100-65411G
DyLight 550
NB100-65411R
DyLight 594
NB100-65411DL594
DyLight 680
NB100-65411FR
DyLight 755
NB100-65411IR
FITC
NB100-65411F
HRP
NB100-65411H
Janelia Fluor 549
NB100-65411JF549
Janelia Fluor 646
NB100-65411JF646
PE
NB100-65411PE
PE/Atto594
NB100-65411PEATT594
PE/Cy5.5
NB100-65411PECY55
PE/Cy7
NB100-65411PECY7
PerCP
NB100-65411PCP
Unconjugated
NB100-65411
mFluor Violet 450 SE
NB100-65411MFV450
mFluor Violet 500 SE
NB100-65411MFV500
mFluor Violet 610 SE
NB100-65411MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-65411
BSA Free
NB100-65411
BSA Free
NB100-65411-0.025mg
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HLA ABC Antibody (YTH862.2) [DyLight 650] Summary

Immunogen
PHA activated human peripheral blood lymphocytes.
Specificity
NB100-65411 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. MHC Class I is expressed by all nucleated cells. Recent studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells (1).
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
HLA-A
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for HLA ABC Antibody (YTH862.2) [DyLight 650]

  • A-10 alpha chain
  • FLJ26655
  • HLA class I histocompatibility antigen, A-1 alpha chain
  • HLA class I histocompatibility antigen, A-28 alpha chain
  • HLA class I histocompatibility antigen, A-9 alpha chain
  • HLAA
  • major histocompatibility complex, class I, A
  • MHC class I antigen A*1
  • MHC class I antigen A*11
  • MHC class I antigen A*80

Background

HLA CLass I is involved in the presentation of foreign antigens to the immune system. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HLA-A