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HIP-55 Antibody

Images

 
Independent Antibodies: Western Blot: HIP-55 Antibody [NBP1-91988] - Analysis using Anti-DBNL antibody NBP1-91988 (A) shows similar pattern to independent antibody NBP1-91989 (B).
Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-91988] - Staining of human testis.
Western Blot: HIP-55 Antibody [NBP1-91988] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-91988] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-91988] - Staining of human lymph node shows high expression.
Orthogonal Strategies: Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-91988] - Staining in human lymph node and liver tissues using anti-DBNL antibody. Corresponding DBNL RNA-seq data are presented for the ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-91988] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-DBNL antibody NBP1-91988 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-91988] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HIP-55 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: INWTGEGVNDVRKGACASHVSTMASFLKGAHVTINARAEEDVEPECIMEKVAKASGANYSFHKESGRFQDVGPQAPVGSVYQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DBNL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
HIP-55 Protein (NBP1-91988PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HIP-55 Antibody

  • ABP1
  • Cervical SH3P7
  • CMAP
  • drebrin-F
  • drebrin-like protein
  • drebrin-like
  • HIP-55SH3P7Cervical mucin-associated protein
  • HPK1-interacting protein of 55 kDa
  • SH3 domain-containing protein 7
  • src homology 3 domain-containing protein HIP-55

Background

HIP55 possesses a SCAD domain that binds to polymerized actin and an SH3 domain. The latter is suggested to bind to Dynamin 1, a protein involved in the mechanism responsible for the formation of endocytotic vesicles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DBNL