![Western Blot: H1F0 Antibody (N/A) [NBP3-07713] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody.](http://images.novusbio.com/fullsize/H1F0-Antibody-N-A-Western-Blot-NBP3-07713-img0004.jpg "Western Blot: H1F0 Antibody (N/A) [NBP3-07713] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08698) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant full-length human H1F0 protein |
| Localization | Nuclear |
| Marker | Pan Nuclear Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H1-0 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H1-0 |
![Immunocytochemistry/Immunofluorescence: H1F0 Antibody (N/A) [NBP3-07713] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody followed by goat anti-rabbit IgG-CF488 (green). Phalloidin is used to stain the membrane.](http://images.novusbio.com/fullsize/H1F0-Antibody-N-A-Immunocytochemistry-Immunofluorescence-NBP3-07713-img0002.jpg "Immunocytochemistry/Immunofluorescence: H1F0 Antibody (N/A) [NBP3-07713] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody followed by goat anti-rabbit IgG-CF488 (green). Phalloidin is used to stain the membrane.")
![Immunohistochemistry-Paraffin: H1F0 Antibody (N/A) [NBP3-07713] - Formalin-fixed, paraffin-embedded human Tonsil stained with H1F0 Rabbit Polyclonal Antibody.](http://images.novusbio.com/fullsize/H1F0-Antibody-N-A-Immunohistochemistry-Paraffin-NBP3-07713-img0003.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (N/A) [NBP3-07713] - Formalin-fixed, paraffin-embedded human Tonsil stained with H1F0 Rabbit Polyclonal Antibody.")
![Flow Cytometry: H1F0 Antibody (N/A) [NBP3-07713] - Flow Cytometric Analysis of HeLa cells. H1F0 antibody followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/H1F0-Antibody-N-A-Flow-Cytometry-NBP3-07713-img0001.jpg "Flow Cytometry: H1F0 Antibody (N/A) [NBP3-07713] - Flow Cytometric Analysis of HeLa cells. H1F0 antibody followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).")
![Western Blot HSPB8/HSP22 Antibody (520817) [Unconjugated]](https://images.novusbio.com/images2/HSPB8_MAB4987_Western_Blot_9457.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")