![Western Blot: H1F0 Antibody [NBP2-16772] - HepG2 whole cell extracts and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (NBP2-16772) at a dilution of 1:10000 and developed with Trident Sharp-ECL.](http://images.novusbio.com/fullsize/H1F0-Antibody-Western-Blot-NBP2-16772-img0015.jpg "Western Blot: H1F0 Antibody [NBP2-16772] - HepG2 whole cell extracts and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (NBP2-16772) at a dilution of 1:10000 and developed with Trident Sharp-ECL.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human H1F0.0. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H1-0 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H1-0 |
![Immunocytochemistry/Immunofluorescence: H1F0 Antibody [NBP2-16772] - Histone H1.0 antibody detects Histone H1.0 protein at nucleus by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H1.0 stained by Histone H1.0 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16772-img0016.jpg "Immunocytochemistry/Immunofluorescence: H1F0 Antibody [NBP2-16772] - Histone H1.0 antibody detects Histone H1.0 protein at nucleus by immunofluorescent analysis.Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H1.0 stained by Histone H1.0 antibody diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0011.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50")
![Western Blot: H1F0 Antibody [NBP2-16772] - Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with Histone H1 antibody diluted by 1:5000.](http://images.novusbio.com/fullsize/H1F0-Antibody-Western-Blot-NBP2-16772-img0012.jpg "Western Blot: H1F0 Antibody [NBP2-16772] - Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with Histone H1 antibody diluted by 1:5000.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0003.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0004.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded rat fore brain. Histone H1.0 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0006.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded rat fore brain. Histone H1.0 antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP2-16772-img0010.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP2-16772] - Paraffin-embedded mouse brain. Histone H1.0 antibody diluted at 1:50")
![Western Blot HSPB8/HSP22 Antibody (520817) [Unconjugated]](https://images.novusbio.com/images2/HSPB8_MAB4987_Western_Blot_9457.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")