![Western Blot: H1F0 Antibody [NBP1-83308] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-2 OS](http://images.novusbio.com/fullsize/H1F0-Antibody-Western-Blot-NBP1-83308-img0004.jpg "Western Blot: H1F0 Antibody [NBP1-83308] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-2 OS")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: MTENSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASK |
| Predicted Species | Mouse (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H1-0 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H1-0 |
![Immunocytochemistry/Immunofluorescence: H1F0 Antibody [NBP1-83308] - Staining of human cell line A-431 shows localization to nucleus & actin filaments.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83308-img0005.jpg "Immunocytochemistry/Immunofluorescence: H1F0 Antibody [NBP1-83308] - Staining of human cell line A-431 shows localization to nucleus & actin filaments.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining in human prostate and lymph node tissues using anti-H1F0 antibody. Corresponding H1F0 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP1-83308-img0008.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining in human prostate and lymph node tissues using anti-H1F0 antibody. Corresponding H1F0 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining of human duodenum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP1-83308-img0002.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining of human duodenum shows strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP1-83308-img0006.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining of human lymph node shows low expression as expected.")
![Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize/H1F0-Antibody-Immunohistochemistry-Paraffin-NBP1-83308-img0007.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody [NBP1-83308] - Staining of human prostate shows high expression.")
![Western Blot HSPB8/HSP22 Antibody (520817) [Unconjugated]](https://images.novusbio.com/images2/HSPB8_MAB4987_Western_Blot_9457.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")