![Western Blot: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (r1415-1).](http://images.novusbio.com/fullsize/H1F0-Antibody-r1415-1-Azide-and-BSA-Free-Western-Blot-NBP2-54303-img0005.jpg "Western Blot: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (r1415-1).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | r1415-1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of recombinant monoclonal antibody purified by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53143). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant full-length human H1F0 protein |
| Localization | Nuclear |
| Marker | Pan Nuclear Marker |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H1-0 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H1-0 |
![Immunocytochemistry/Immunofluorescence: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.](http://images.novusbio.com/fullsize/H1F0-Antibody-r1415-1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54303-img0003.jpg "Immunocytochemistry/Immunofluorescence: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.")
![Immunohistochemistry-Paraffin: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1)](http://images.novusbio.com/fullsize/H1F0-Antibody-r1415-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54303-img0001.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1)")
![Flow Cytometry: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Flow Cytometric Analysis of HeLa cells. H1F0 Mouse Recombinant Monoclonal Antibody (r1415-1) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/H1F0-Antibody-r1415-1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54303-img0004.jpg "Flow Cytometry: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Flow Cytometric Analysis of HeLa cells. H1F0 Mouse Recombinant Monoclonal Antibody (r1415-1) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1)](http://images.novusbio.com/fullsize/H1F0-Antibody-r1415-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54303-img0002.jpg "Immunohistochemistry-Paraffin: H1F0 Antibody (r1415-1) - Azide and BSA Free [NBP2-54303] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1)")
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