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GRK3 Antibody (497007) [Unconjugated]

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # ...read more
GRK3 was detected in immersion fixed CEM-NKr human Natural Killer cell resistant T-lymphoblastoid cell line using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 8 µg/mL for 3 hours at room ...read more
GRK3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 5 µg/mL for 1 hour at room temperature ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
497007
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB47851-SP
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GRK3 Antibody (497007) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GRK3
Pro469-Leu688
Accession # P35626
Specificity
Detects human GRK3 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRK3 Antibody (497007) [Unconjugated]

  • ADRBK2
  • adrenergic, beta, receptor kinase 2
  • BARK2
  • BARK2beta-adrenergic receptor kinase 2
  • Beta-ARK-2
  • EC 2.7.11
  • EC 2.7.11.15
  • G-protein-coupled receptor kinase 3
  • GRK3
  • GRK3Beta-ARK-2

Background

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK3 (also known as beta -adrenergic receptor kinase 2, or beta ARK2) and GRK2 ( beta ARK1) are members of the beta -adrenergic receptor kinase subfamily. GRK3 may phosphorylate alpha -synuclein at S129, suggesting a potential involvement for this kinase in Parkinson’s disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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