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Rat GRK2 ELISA Kit (Colorimetric)

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ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] - Samples were spiked with high concentrations of Rat GRK2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] - Samples were spiked with high concentrations of Rat GRK2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Rat GRK2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat GRK2 in samples. No significant cross-reactivity or interference between Rat GRK2 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.87%
Intra-Assay
CV% < 5.37%
Spike Recovery
85-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GRK2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat GRK2 ELISA Kit (Colorimetric)

  • ADRBK1
  • adrenergic, beta, receptor kinase 1
  • BARK
  • BARK1
  • BARK1beta-ARK-1
  • Beta-ARK1
  • Beta-ARK-1
  • EC 2.7.11
  • EC 2.7.11.15
  • FLJ16718
  • G-protein coupled receptor kinase 2
  • GRK2
  • GRK2beta-adrenergic receptor kinase 1

Background

Adrenergic Receptor beta Kinase 1 (ADRBK1), also known as GRK2, is a member of the G-protein receptor kinase family. A ubiquitous cytosolic enzyme, GRK-2 specifically phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed under high agonist concentrations. Defects in GRK2 coupling with G-protein receptors may lead to heart failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Rat GRK2 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol GRK2