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GCLC Antibody (OTI1A3) - Azide and BSA Free

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Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.
Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLC.
Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more
Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GCLC Antibody (OTI1A3) - Azide and BSA Free [NBP2-70830] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC
Clone
OTI1A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70830AF350
Alexa Fluor 405
NBP2-70830AF405
Alexa Fluor 488
NBP2-70830AF488
Alexa Fluor 532
NBP2-70830AF532
Alexa Fluor 594
NBP2-70830AF594
Alexa Fluor 647
NBP2-70830AF647
Alexa Fluor 700
NBP2-70830AF700
Alexa Fluor 750
NBP2-70830AF750
Allophycocyanin
NBP2-70830APC
Biotin
NBP2-70830B
CoraFluor 1
NBP2-70830CL1
DyLight 350
NBP2-70830UV
DyLight 405
NBP2-70830V
DyLight 488
NBP2-70830G
DyLight 550
NBP2-70830R
DyLight 594
NBP2-70830DL594
DyLight 650
NBP2-70830C
DyLight 680
NBP2-70830FR
DyLight 755
NBP2-70830IR
FITC
NBP2-70830F
HRP
NBP2-70830H
Janelia Fluor 525
NBP2-70830JF525
Janelia Fluor 549
NBP2-70830JF549
Janelia Fluor 585
NBP2-70830JF585
Janelia Fluor 635
NBP2-70830JF635
Janelia Fluor 646
NBP2-70830JF646
Janelia Fluor 669
NBP2-70830JF669
PE
NBP2-70830PE
PerCP
NBP2-70830PCP
Unconjugated
NBP2-45830
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45830
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GCLC Antibody (OTI1A3) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human GCLC(NP_001489) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GCLC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:200-1000
Theoretical MW
72.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GCLC Antibody (OTI1A3) - Azide and BSA Free

  • EC 6.3.2.2
  • Gamma-ECS
  • Gamma-glutamylcysteine synthetase
  • GCLC
  • GCS heavy chain
  • GCS
  • GLCL
  • GLCLC
  • GLCLCGCL
  • glutamate--cysteine ligase catalytic subunit
  • glutamate-cysteine ligase, catalytic subunit

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GCLC