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FUS Antibody (HL2454) - Azide and BSA Free

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Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (NBP3-25471) diluted at ...read more
Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - FUS antibody [HL2454] detects FUS protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. FUS stained by ...read more
Western Blot: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FUS antibody [HL2454] (NBP3-25471) diluted at ...read more
Genetic Strategies: Knockout Validated: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - Wild-type (WT) and FUS knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane ...read more
Immunohistochemistry-Paraffin: FUS Antibody (HL2454) - Azide and BSA Free [NBP3-25471] - FUS antibody [HL2454] detects FUS protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. FUS stained by ...read more
ICC/IF-FUS Antibody (HL2454) - Azide and BSA Free-NBP3-25471-HepG2 cells were fixed in 4% PFA.Green: FUS stained by FUS antibody [HL2454]diluted at 1:500.Blue: Fluoroshield with DAPI.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
HL2454
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Validated by:
     

Genetic Strategies

   

Order Details

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FUS Antibody (HL2454) - Azide and BSA Free Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment of human FUS.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FUS
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for FUS Antibody (HL2454) - Azide and BSA Free

  • 75 kDa DNA-pairing protein
  • ALS6
  • amyotrophic lateral sclerosis 6
  • FUS1
  • fused in sarcoma
  • fusion (involved in t(12;16) in malignant liposarcoma)
  • fusion gene in myxoid liposarcoma
  • fusion, derived from t(12;16) malignant liposarcoma
  • fus-like protein
  • heterogeneous nuclear ribonucleoprotein P2
  • HNRNPP2
  • Oncogene FUS
  • Oncogene TLS
  • POMP75
  • RNA-binding protein FUS
  • TLShnRNP-P2
  • Translocated in liposarcoma protein

Background

FUS, fused in sarcoma, encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FUS Antibody (NBP3-25471) (0)

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Secondary Antibodies

 

Isotype Controls

Additional FUS Products

Research Areas for FUS Antibody (NBP3-25471)

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Blogs on FUS.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Amyotrophic Lateral Sclerosis Infographic
Amyotrophic lateral sclerosis is a neurological disease which impacts motor neurons that are involved in muscle movement throughout the body. The progressive degeneration of neurons causes weakened muscles and can lead to paralysis. There is no cure f...  Read full blog post.

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Bioinformatics

Gene Symbol FUS