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FARSLA Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Analysis in human cerebral cortex and liver tissues. Corresponding FARSA RNA-seq data are presented for the same tissues.
Western Blot: FARSLA Antibody [NBP1-88067] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: FARSLA Antibody [NBP1-88067] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Western Blot: FARSLA Antibody [NBP1-88067] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human cerebral cortex shows very strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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FARSLA Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FARSA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FARSLA Protein (NBP1-88067PEP)
Publications
Read Publication using NBP1-88067.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FARSLA Antibody

  • CML33pheRS
  • EC 6.1.1.20
  • FARS
  • FARSL
  • FARSLA
  • FRSA
  • PheHA
  • phenylalanine tRNA ligase 1, alpha, cytoplasmic
  • Phenylalanine--tRNA ligase alpha chain
  • phenylalanine-tRNA synthetase alpha-subunit
  • phenylalanine-tRNA synthetase-like
  • phenylalanine-tRNA synthetase-like, alpha subunit
  • phenylalanyl-tRNA synthetase alpha chain
  • phenylalanyl-tRNA synthetase, alpha subunit
  • phenylalanyl-tRNA synthetase-like, alpha subunit
  • PheRS

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FARSLA Antibody (NBP1-88067)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FARSLA Antibody (NBP1-88067) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FARSA