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FABP1/L-FABP Antibody [Unconjugated]

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Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line, mouse liver tissue, human liver tissue, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/ Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line, mouse liver tissue, human liver tissue, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/ ...read more
Western blot shows lysate of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/ Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by ...read more
FABP1 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Human/Mouse/Rat FABP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of H4‑II‑E‑C3 rat hepatoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1565-SP
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FABP1/L-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat FABP1/L-FABP
Met1-Ile127
Accession # P02692
Specificity
Detects rat FABP1/L-FABP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.2-0.5 ug/mL
Publications
Read Publications using
AF1565 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP1/L-FABP Antibody [Unconjugated]

  • FABP1
  • FABPL
  • fatty acid binding protein 1, liver
  • fatty acid-binding protein, liver
  • LFABP
  • L-FABP
  • L-FABPFatty acid-binding protein 1
  • Liver-type fatty acid-binding protein

Background

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene, and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney, and lung. FABP1 binds free fatty acids, their co-enzyme A derivatives, and may be involved in intracellular lipid transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP1/L-FABP Antibody (AF1565)(3)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 2 applications: Immunohistochemistry, Western Blot.


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Immunohistochemistry
(1)
Western Blot
(1)
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(1)
Transgenic Mouse
(1)
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Bioinformatics

Gene Symbol FABP1
Uniprot