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Eps8 Antibody

Images

 
Western Blot: Eps8 Antibody [NB300-819] - (1ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] - Negative Control showing staining of paraffin embedded Human Pancreas, with no primary antibody.
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] - (8ug/ml) staining of paraffin embedded Human Pancreas. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] - (6ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Eps8 Antibody Summary

Immunogen
Peptide with sequence C-SGVESFDEGSSH corresponding to C-Terminus according to NP_004438.3.
Predicted Species
Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPS8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6-8 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1-3 ug/ml
Application Notes
WB: Approx. 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 91.9kDa according to NP_004438.3). An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
Control
Eps8 Overexpression Lysate
Publications
Read Publication using NB300-819.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Eps8 Antibody

  • epidermal growth factor receptor kinase substrate 8
  • epidermal growth factor receptor pathway substrate 8
  • Eps8

Background

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Eps8 Antibody (NB300-819)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Eps8 Antibody (NB300-819) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EPS8
Entrez
Uniprot