EphB4 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin‑B2 and ephrin‑B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate - Chlorocebus aethiops (African Green Monkey), Zebrafish.

We have publications tested in 8 applications: Bioassay, Flow Cytometry, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 52. Show All 52 Publications. Collapse Publications.

Publications using AF446	Applications	Species
Chen, D;Wiggins, D;Sevick, EM;Davis, MJ;King, PD; An EPHB4-RASA1 signaling complex inhibits shear stress-induced Ras-MAPK activation in lymphatic endothelial cells to promote the development of lymphatic vessel valves bioRxiv : the preprint server for biology 2023-11-23 [PMID: 38045382] (Western Blot, Human)	Western Blot	Human
Zhao, S;Mekbib, KY;van der Ent, MA;Allington, G;Prendergast, A;Chau, JE;Smith, H;Shohfi, J;Ocken, J;Duran, D;Furey, CG;Hao, LT;Duy, PQ;Reeves, BC;Zhang, J;Nelson-Williams, C;Chen, D;Li, B;Nottoli, T;Bai, S;Rolle, M;Zeng, X;Dong, W;Fu, PY;Wang, YC;Mane, S;Piwowarczyk, P;Fehnel, KP;See, AP;Iskandar, BJ;Aagaard-Kienitz, B;Moyer, QJ;Dennis, E;Kiziltug, E;Kundishora, AJ;DeSpenza, T;Greenberg, ABW;Kidanemariam, SM;Hale, AT;Johnston, JM;Jackson, EM;Storm, PB;Lang, SS;Butler, WE;Carter, BS;Chapman, P;Stapleton, CJ;Patel, AB;Rodesch, G;Smajda, S;Berenstein, A;Barak, T;Erson-Omay, EZ;Zhao, H;Moreno-De-Luca, A;Proctor, MR;Smith, ER;Orbach, DB;Alper, SL;Nicoli, S;Boggon, TJ;Lifton, RP;Gunel, M;King, PD;Jin, SC;Kahle, KT; Mutation of key signaling regulators of cerebrovascular development in vein of Galen malformations Nature communications 2023-11-17 [PMID: 37978175] (Immunoprecipitation, Primate - Chlorocebus aethiops (African Green Monkey))	Immunoprecipitation	Primate - Chlorocebus aethiops (African Green Monkey)
S Zhao, KY Mekbib, MA van der En, G Allington, A Prendergas, JE Chau, H Smith, J Shohfi, J Ocken, D Duran, CG Furey, HT Le, PQ Duy, BC Reeves, J Zhang, C Nelson-Wil, D Chen, B Li, T Nottoli, S Bai, M Rolle, X Zeng, W Dong, PY Fu, YC Wang, S Mane, P Piwowarczy, KP Fehnel, AP See, BJ Iskandar, B Aagaard-Ki, AJ Kundishora, T DeSpenza, ABW Greenberg, SM Kidanemari, AT Hale, JM Johnston, EM Jackson, PB Storm, SS Lang, WE Butler, BS Carter, P Chapman, CJ Stapleton, AB Patel, G Rodesch, S Smajda, A Berenstein, T Barak, EZ Erson-Omay, H Zhao, A Moreno-De-, MR Proctor, ER Smith, DB Orbach, SL Alper, S Nicoli, TJ Boggon, RP Lifton, M Gunel, PD King, SC Jin, KT Kahle Genetic dysregulation of an endothelial Ras signaling network in vein of Galen malformations bioRxiv : the preprint server for biology, 2023-03-21;0(0):. 2023-03-21 [PMID: 36993588] (Immunoprecipitation, Zebrafish)	Immunoprecipitation	Zebrafish
S Kohara, K Ogawa Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 2022-12-12;10(12):. 2022-12-12 [PMID: 36551990] (Bioassay, Mouse)	Bioassay	Mouse
H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa Histochemical examination of blood vessels in murine femora with intermittent PTH administration Journal of oral biosciences, 2022-05-15;0(0):. 2022-05-15 [PMID: 35584768] (IHC, Mouse)	IHC	Mouse
Victor H. Guaiquil, Cissy Xiao, Daniel Lara, Greigory Dimailig, Qiang Zhou Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury Experimental Eye Research 6/1/2022 [PMID: 35427568]
AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022-03-15;38(11):110510. 2022-03-15 [PMID: 35294885] (IF, Mouse)	IF	Mouse
J Alam, K Ogawa Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary Reproductive biology, 2021-05-13;21(3):100511. 2021-05-13 [PMID: 33991765] (IHC, Mouse)	IHC	Mouse
C Donato, L Kunz, F Castro-Gin, A Paasinen-S, K Strittmatt, BM Szczerba, R Scherrer, N Di Maggio, W Heusermann, O Biehlmaier, C Beisel, M Vetter, C Rochlitz, WP Weber, A Banfi, T Schroeder, N Aceto Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 2020-09-08;32(10):108105. 2020-09-08 [PMID: 32905777] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams Endothelial EphB4 maintains vascular integrity and transport function in adult heart Elife, 2019-11-29;8(0):. 2019-11-29 [PMID: 31782728] (Western Blot, Human)	Western Blot	Human
S Payne, M Gunadasa-R, A Neal, AN Redpath, J Patel, KM Chouliaras, I Ratnayaka, N Smart, S De Val Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart Nat Commun, 2019-07-22;10(1):3276. 2019-07-22 [PMID: 31332177] (IHC-Fr, Mouse)	IHC-Fr	Mouse
S Bhatia, A Oweida, S Lennon, LB Darragh, D Milner, AV Phan, AC Mueller, B Van Court, D Raben, NJ Serkova, XJ Wang, A Jimeno, ET Clambey, EB Pasquale, SD Karam Inhibition of EphB4-ephrin-B2 signaling reprograms the tumor immune microenvironment in head and neck cancers Cancer Res., 2019-03-20;0(0):. 3/20/2019 [PMID: 30894369]
A Neal, S Nornes, S Payne, MD Wallace, M Fritzsche, P Louphrasit, RN Wilkinson, KM Chouliaras, K Liu, K Plant, R Sholapurka, I Ratnayaka, W Herzog, G Bond, T Chico, G Bou-Ghario, S De Val Venous identity requires BMP signalling through ALK3 Nat Commun, 2019-01-28;10(1):453. 2019-01-28 [PMID: 30692543] (IHC, IHC-P, Mouse)	IHC, IHC-P	Mouse
Christian Uhl, Moritz Markel, Thomas Broggini, Melina Nieminen, Irina Kremenetskaia, Peter Vajkoczy et al. EphB4 mediates resistance to antiangiogenic therapy in experimental glioma Angiogenesis 11/1/2018 [PMID: 29987450]
Roxana Ola, Sandrine H. Künzel, Feng Zhang, Gael Genet, Raja Chakraborty, Laurence Pibouin-Fragner et al. SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2 Circulation 11/20/2018 [PMID: 29976569]
E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 2018-04-11;0(0):. 2018-04-11 [PMID: 29643120] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Max A. Tischfield, Caroline D. Robson, Nicole M. Gilette, Shek Man Chim, Folasade A. Sofela, Michelle M. DeLisle et al. Cerebral Vein Malformations Result from Loss of Twist1 Expression and BMP Signaling from Skull Progenitor Cells and Dura Developmental Cell 9/1/2017 [PMID: 28844842]
H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice Biomed. Res., 2017-01-01;38(2):123-134. 2017-01-01 [PMID: 28442663] (IHC, Mouse)	IHC	Mouse
Man Chu, Taotao Li, Bin Shen, Xudong Cao, Haoyu Zhong, Luqing Zhang et al. Angiopoietin receptor Tie2 is required for vein specification and maintenance via regulating COUP-TFII eLife 12/22/2016 [PMID: 28005008]
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016-07-29;7(0):12329. 2016-07-29 [PMID: 27470974] (Western Blot, Mouse)	Western Blot	Mouse
Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol J Exp Clin Cancer Res, 2016-07-22;35(1):118. 2016-07-22 [PMID: 27448598] (IHC-P, Mouse)	IHC-P	Mouse
EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis J Clin Invest, 2016-07-11;0(0):. 2016-07-11 [PMID: 27400125] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Bai H, Wang M, Foster TR et al. Pericardial patch venoplasty heals via attraction of venous progenitor cells Physiol Rep 6/1/2016 [PMID: 27354544]
Omanma Adighibe, Russell D. Leek, Marta Fernandez-Mercado, Jiangting Hu, Cameron Snell, Kevin C. Gatter et al. Why some tumours trigger neovascularisation and others don’t: the story thus far Chinese Journal of Cancer 12/1/2016 [PMID: 26873439]
M Wang, MJ Collins, TR Foster, H Bai, T Hashimoto, JM Santana, C Shu, A Dardik Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase J. Vasc. Surg., 2016-01-24;65(1):179-189. 2016-01-24 [PMID: 26817610] (Western Blot, Mouse)	Western Blot	Mouse
Elly Sinkala, Amy E. Herr Single-Cell Western Blotting Methods in Molecular Biology 11/7/2015 [PMID: 26542711]
Beatriz Morancho, Águeda Martínez-Barriocanal, Josep Villanueva, Joaquín Arribas Role of ADAM17 in the non-cell autonomous effects of oncogene-induced senescence Breast Cancer Research 12/1/2015 [PMID: 26260680]
Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance. Stem Cells, 2015-06-23;33(9):2838-49. 2015-06-23 [PMID: 26033476] (Western Blot, Mouse)	Western Blot	Mouse
Johanna Liebl, Siwei Zhang, Markus Moser, Yan Agalarov, Cansaran Saygili Demir, Bianca Hager et al. Cdk5 controls lymphatic vessel development and function by phosphorylation of Foxc2 Nature Communications 6/1/2015 [PMID: 26027726]
Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Western Blot, Mouse)	Western Blot	Mouse
Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cells World Journal of Gastroenterology 1/14/2015 [PMID: 25593460]
Valverde-Franco G, Hum D, Matsuo K, Lussier B, Pelletier J, Fahmi H, Kapoor M, Martel-Pelletier J The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice. Am J Pathol, 2014-11-29;185(2):335-46. 2014-11-29 [PMID: 25453723] (IHC-P, Mouse)	IHC-P	Mouse
Hughes A, Spelke D, Xu Z, Kang C, Schaffer D, Herr A Single-cell western blotting. Nat Methods, 2014-06-01;11(7):749-55. 2014-06-01 [PMID: 24880876] (Western Blot, Rat)	Western Blot	Rat
Lindskog , Henrik, Kim , Yung Hae, Jelin , Eric B, Kong , Yupeng, Guevara-Gallardo , Salvador, Kim , Tyson N, Wang , Rong A Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals. Development, 2014-03-01;141(5):1120-8. 2014-03-01 [PMID: 24550118] (IHC, Mouse)	IHC	Mouse
Lange A, Haitchi H, LeCras T, Sridharan A, Xu Y, Wert S, James J, Udell N, Thurner P, Whitsett J Sox17 is required for normal pulmonary vascular morphogenesis. Dev Biol, 2014-01-10;387(1):109-20. 2014-01-10 [PMID: 24418654] (IHC-P, Mouse)	IHC-P	Mouse
Karl Degenhardt, Manvendra K Singh, Haig Aghajanian, Daniele Massera, Qiaohong Wang, Jun Li et al. Semaphorin 3d signaling defects are associated with anomalous pulmonary venous connections Nature Medicine 6/1/2013 [PMID: 23685842]
Reema B. Davis, Carol D. Curtis, Courtney T. Griffin BRG1 promotes COUP-TFII expression and venous specification during embryonic vascular development Development 3/15/2013 [PMID: 23406903]
Stephanie J. Munger, John D. Kanady, Alexander M. Simon Absence of venous valves in mice lacking Connexin37 Developmental Biology 1/15/2013 [PMID: 23142761]
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012-05-23;42(6):1562-72. 2012-05-23 [PMID: 22622783] (Immunoprecipitation, Western Blot, Mouse)	Immunoprecipitation, Western Blot	Mouse
Muto A, Yi T, Harrison KD, Davalos A, Fancher TT, Ziegler KR, Feigel A, Kondo Y, Nishibe T, Sessa WC, Dardik A Eph-B4 prevents venous adaptive remodeling in the adult arterial environment. J. Exp. Med., 2011-02-21;208(3):561-75. 2011-02-21 [PMID: 21339325] (IHC, Mouse)	IHC	Mouse
Ni A, Lashnits E, Yao LC et al. Rapid remodeling of airway vascular architecture at birth. Dev Dyn 9/1/2010 [PMID: 20730909]
Foster KE, Gordon J, Cardenas K EphB-ephrin-B2 interactions are required for thymus migration during organogenesis. Proc. Natl. Acad. Sci. U.S.A., 2010-07-08;107(30):13414-9. 2010-07-08 [PMID: 20616004] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Wang Y, Nakayama M, Pitulescu ME, Schmidt TS, Bochenek ML, Sakakibara A, Adams S, Davy A, Deutsch U, Luthi U, Barberis A, Benjamin LE, Makinen T, Nobes CD, Adams RH Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. Nature, 2010-05-27;465(7297):483-6. 2010-05-27 [PMID: 20445537] (IHC-P, Mouse)	IHC-P	Mouse
Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 2010-04-01;120(5):1694-707. 2010-04-01 [PMID: 20364082] (IHC-P, Mouse)	IHC-P	Mouse
Xie J, Wu T, Xu K, Huang IK, Cleaver O, Huang CL Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice. Am. J. Pathol., 2009-07-30;175(3):1315-27. 2009-07-30 [PMID: 19644017] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Kim YH, Hu H, Guevara-Gallardo S, Lam MT, Fong SY, Wang RA Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis. Development, 2008-11-01;135(22):3755-64. 2008-11-01 [PMID: 18952909] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Allan EH, Hausler KD, Wei T, Gooi JH, Quinn JM, Crimeen-Irwin B, Pompolo S, Sims NA, Gillespie MT, Onyia JE, Martin TJ EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts. J. Bone Miner. Res., 2008-08-01;23(8):1170-81. 2008-08-01 [PMID: 18627264] (Western Blot, Mouse)	Western Blot	Mouse
Zhang J, Dong H, Wang B, Zhu S, Croy BA Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice. Biol. Reprod., 2008-05-07;79(3):450-8. 2008-05-07 [PMID: 18463357] (IHC-P, Mouse)	IHC-P	Mouse
Yang NY, Pasquale EB, Owen LB, Ethell IM The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization. J. Biol. Chem., 2006-08-31;281(43):32574-86. 2006-08-31 [PMID: 16950769] (Flow Cytometry, Immunoprecipitation, Western Blot, Mouse)	Flow Cytometry, Immunoprecipitation, Western Blot	Mouse
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 2006-02-01;119(0):559-70. 2006-02-01 [PMID: 16443753] (IHC, Mouse)	IHC	Mouse
Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG Inhibition of tumor growth and angiogenesis by soluble EphB4. Neoplasia, 2004-05-01;6(3):248-57. 2004-05-01 [PMID: 15153337] (Western Blot, Human)	Western Blot	Human
Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J. Cell. Sci., 2003-05-06;116(0):2461-70. 2003-05-06 [PMID: 12734395] (Western Blot, Human)	Western Blot	Human
Show All 52 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Rat	01/08/2015	View Summary Application Western Blot Sample Tested See PMID 22398409 Species Rat
	4 reviewed by: Verified Customer	IF	Rat	01/08/2015	View Summary Application Immunofluorescence Sample Tested See PMID 22398409 Species Rat

Array AF446

• EphB4 Antibodies
• EphB4 Lysate
• EphB4 ELISA
• EphB4 Proteins and Enzymes