Endothelial Lipase Antibody

Endothelial lipase (EL) is an essential element of HDL metabolism which catalyzes the hydrolysis of HDL-phospholipids for facilitating their clearance from circulation. EL is synthesized in endothelial cells, however, is also expressed in other cell types such as coronary artery endothelial cells, umbilical vein endothelial cells, hepatocytes, osteosarcoma cell lines etc. with highest levels seen in organs including liver, placenta, lung, thyroid, kidney, testis and corpus luteum of ovary. EL is synthesized as a 55 kD protein, secreted as a 68 kD mature protein and after secretion, a portion of EL is cleaved and inactivated by convertases. Uncleaved EL is bound to heparan-sulfate proteoglycans on the cell surfaces where via its bridging functions, it facilitates HDL particle binding/uptake and selective uptake of HDL cholesteryl esters. Through its marked sn-1 phospholipase activity, EL cleaves lipoprotein-associated phospholipids, primarily HDL-phosphatidylethanolamine (HDL-PE) and -phosphatidylcholine (HDL-PC), generating mostly saturated free fatty acids and sn-1 lyso sn-2 acyl lysophospholipids (LysoPL). By its rather weak lysophospholipase activity, EL further hydrolyzes some of those EL-generated LPL, liberating unsaturated FFA. EL expression increases in cultured endothelial cells exposed to interleukin-1 beta or TNF-alpha as well as in vivo upon LPS exposure suggesting its role in inflammatory conditions. Accordingly, an elevated EL levels are found in patients with subclinical inflammation as encountered in the metabolic syndrome and coronary atherosclerosis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.

Showing Publications 1 - 6 of 6.

Publications using NB400-111	Applications	Species
Yang Y, Kuwano T, Lagor WR et al. Lipidomic analyses of female mice lacking hepatic lipase and endothelial lipase indicate selective modulation of plasma lipid species. Lipids. 2014-06-01 [PMID: 24777581] (WB, Human)	WB	Human
Robert J, Weber B, Frese L et al. A Three-Dimensional Engineered Artery Model for In Vitro Atherosclerosis Research. PLoS One. 2013-11-14 [PMID: 24244566] (IHC-Fr, ICC/IF, Human)	IHC-Fr, ICC/IF	Human
Essaji Y, Yang Y, Albert CJ et al. Hydrolysis Products Generated by Lipoprotein Lipase and Endothelial Lipase Differentially Impact THP-1 Macrophage Cell Signalling Pathways. Lipids 2013-06-22 [PMID: 23794138] (WB, Human)	WB	Human
Razzaghi H, Tempczyk-Russell A, Haubold K et al. Genetic and Structure-Function Studies of Missense Mutations in Human Endothelial Lipase PLoS One 2013-01-01 [PMID: 23536757] (WB, Human)	WB	Human
Wiersma H, Gatti A, Nijstad N et al. Hepatic SR-BI, not endothelial lipase, expression determines biliary cholesterol secretion in mice. J Lipid Res;50(8):1571-1580. 2009-01-01 [PMID: 19252221]
Nijstad N, Wiersma H, Gautier T et al. Scavenger Receptor BI-mediated Selective Uptake Is Required for the Remodeling of High Density Lipoprotein by Endothelial Lipase. J Biol Chem;284(10):6093-6100. 2009-01-01 [PMID: 19136670] (WB, Human)	WB	Human

Array NB400-111

• Endothelial Lipase Antibodies
• Endothelial Lipase Lysate
• Endothelial Lipase Proteins