Home » EMMPRIN/CD147 » EMMPRIN/CD147 Antibodies » EMMPRIN/CD147 Antibody (109403) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

EMMPRIN/CD147 Antibody (109403) [Unconjugated]

 (10 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EMMPRIN/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ...read more
Recombinant Human EMMPRIN/CD147 protein was serially diluted 2-fold and captured by Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human ...read more
Silencing CD147 inhibits HCMV entry into endothelial cells. (A) Lysates from a control endothelial cell line (tAECs) transduced with an empty lentivirus vector (Ctl) or an endothelial cell line (tAECs) transduced with a ...read more
Silencing CD147 inhibits HCMV entry into endothelial cells. (A) Lysates from a control endothelial cell line (tAECs) transduced with an empty lentivirus vector (Ctl) or an endothelial cell line (tAECs) transduced with a ...read more
Clone 10 binding efficiency in human cells and tissue.(A) Using fluorescence-activated cell sorting (FACS) analysis, human fetal astrocytes stained for glial fibrillary acidic protein (GFAP) were additionally stained ...read more
Clone 10 binds most efficiently to extracellular matrix metalloproteinase inducer (EMMPRIN) in western blots. Hybridoma supernatants from several clones are used in a western blot to detect 5 μg of recombinant human ...read more
Clone 10 binding efficiency in human cells and tissue.(A) Using fluorescence-activated cell sorting (FACS) analysis, human fetal astrocytes stained for glial fibrillary acidic protein (GFAP) were additionally stained ...read more
Inhibition of HCMV entry via anti-CD147 antibodies. (A) Human ARPE-19 epithelial cells were left untreated (No Ab) or were pretreated with MAbs specific for the transferrin receptor (TfR) or CD147 (MAbs 9B10, M61, ...read more
HCMV colocalizes with CD147 in early endosomes. ARPE-19 epithelial cells on glass slides were transduced with a baculovirus expressing a fluorescent-tagged early endosome marker, EEA-1. The cells were then incubated ...read more
HCMV colocalizes with CD147 patches on the surface of ARPE-19 cells. (A) ARPE-19 cells on glass slides were incubated with anti-CD147 MAb 109403 and HCMV UL32-GFP virus particles at 4° for 1 h, then shifted to 37° ...read more
Effects of soluble CD147 on HCMV entry and binding to soluble HCMV glycoproteins. (A) A soluble form of CD147 that was fused to a polyhistidine epitope tag and purified from the tissue culture supernatant of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, KO
Clone
109403
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB972-SP
Carrier Free
MAB972-500
100% Guarantee on every product

EMMPRIN/CD147 Antibody (109403) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147
Ala22-His205
Accession # Q54A51
Specificity
Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse EMMPRIN is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BSG
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Knockout Validated
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
MAB972 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMMPRIN/CD147 Antibody (109403) [Unconjugated]

  • 5F7
  • basigin (Ok blood group)
  • Basigin
  • BSG
  • CD147 antigen
  • CD147
  • Collagenase stimulatory factor
  • EMMPRIN
  • EMMPRINTCSF
  • Extracellular matrix metalloproteinase inducer
  • Leukocyte activation antigen M6
  • M6
  • OK blood group antigen
  • OK
  • Tumor cell-derived collagenase stimulatory factor

Background

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily. It is a multifunctional protein involved in reproduction, neural function, inflammation and tumor invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MMP-2 [HRP]
MMP200
N/A MMP-2 [HRP]
N/A MMP-2 [HRP]
(99 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(219 Publications)
Species: Hu
Applications: ELISA
MCT1/SLC16A1
Antibody - BSA Free
NBP1-59656
Western Blot MCT1/SLC16A1 Antibody - BSA Free
Knockdown Validated MCT1/SLC16A1 Antibody - BSA Free
Western Blot MCT1/SLC16A1 Antibody - BSA Free
(14 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(672 Publications)
Species: Hu
Applications: ELISA
MMP-1 Antibody
(36665) [Unconjugated
MAB901
Knockout Validated MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
(39 Publications)
Species: Hu
Applications: IHC, IP, KO, Neut, WB
MMP-14/MT1-MMP
Antibody (128527) [Un ...
MAB9181
Flow Cytometry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]
Immunohistochemistry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]
(11 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, WB
SLC16A4 Antibody
NBP2-13315
Immunohistochemistry-Paraffin SLC16A4 Antibody
Immunohistochemistry-Paraffin SLC16A4 Antibody
Immunohistochemistry-Paraffin SLC16A4 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
MCTS1 Antibody
(OTI2F9)
NBP2-03626
Western Blot MCTS1 Antibody (OTI2F9)
Immunohistochemistry-Paraffin MCTS1 Antibody (OTI2F9)
Immunohistochemistry-Paraffin MCTS1 Antibody (OTI2F9)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Chymase/CMA1/Mast
Cell Chymase Antibody ...
MAB4099
Western Blot Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]
ELISA Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]
(2 Reviews)
(3 Publications)
Species: Hu
Applications: IP, WB
SLC16A3 Antibody
NBP1-81251
Immunocytochemistry/ Immunofluorescence SLC16A3 Antibody
Immunohistochemistry-Paraffin SLC16A3 Antibody
Immunohistochemistry-Paraffin SLC16A3 Antibody
(1 Review)
(6 Publications)
Species: Hu, Mu
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Muscarinic
Acetylcholine Recepto ...
NBP1-87466
Immunohistochemistry-Paraffin Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody
Immunohistochemistry-Paraffin Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody
Immunocytochemistry/ Immunofluorescence Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
EMMPRIN/CD147
Antibody (109403) [Un ...
MAB972
EMMPRIN/CD147 Antibody (109403) [Unconjugated]
Species: Hu
Applications: WB, ELISA, KO

Publications for EMMPRIN/CD147 Antibody (MAB972)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: ELISA Development, Flow Cytometry, ICC, IHC-P, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(1)
ICC
(2)
IHC-P
(2)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB972 Applications Species
Lau, HC;Passalacqua, I;Jung, JH;Kwon, Y;Zocco, D;Park, SS;Oh, SW; Unraveling the surface marker signature of cell-derived vesicles via proteome analysis and nanoparticle flow cytometry Scientific reports 2024-01-02 [PMID: 38167556] (Western Blot, Human) Western Blot Human
P Wang, X Hao, X Li, Y Yan, W Tian, L Xiao, Z Wang, J Dong Curcumin inhibits adverse psychological stress-induced proliferation and invasion of glioma cells via down-regulating the ERK/MAPK pathway Journal of Cellular and Molecular Medicine, 2021-06-25;0(0):. 2021-06-25 [PMID: 34169637] (Western Blot, Human) Western Blot Human
M Aoki, K Koga, M Miyazaki, M Hamasaki, N Koshikawa, M Oyama, H Kozuka-Hat, M Seiki, BP Toole, K Nabeshima CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts BMC Cancer, 2019-09-12;19(1):912. 2019-09-12 [PMID: 31510956] (ICC, IHC-P, Proximity Ligation Assay (PLA), Western Blot, Human) ICC, IHC-P, Proximity Ligation Assay (PLA), Western Blot Human
AP Andersen, J Samsøe-Pet, EK Oernbo, E Boedtkjer, JMA Moreira, M Kveiborg, SF Pedersen The net acid extruders NHE1, NBCn1 and MCT4 promote mammary tumor growth through distinct but overlapping mechanisms Int. J. Cancer, 2018-02-07;0(0):. 2018-02-07 [PMID: 29363134] (Western Blot, Human) Western Blot Human
Ibtissam Marchiq, Jean Albrengues, Sara Granja, Cédric Gaggioli, Jacques Pouysségur, Marie-Pierre Simon Knock out of the BASIGIN/CD147 chaperone of lactate/H+ symporters disproves its pro-tumour action via extracellular matrix metalloproteases (MMPs) induction Oncotarget 9/22/2015 [PMID: 26284589]
Sara Granja, Ibtissam Marchiq, Renaud Le Le Floch, Conceição Souto Moura, Fátima Baltazar, Jacques Pouysségur Disruption of BASIGIN decreases lactic acid export and sensitizes non-small cell lung cancer to biguanides independently of the LKB1 status Oncotarget 3/30/2015 [PMID: 25894929]
Smriti M Agrawal, Claudia Silva, Janet Wang, Jade Pui-Wai Tong, V Wee Yong A novel anti-EMMPRIN function-blocking antibody reduces T cell proliferation and neurotoxicity: relevance to multiple sclerosis Journal of Neuroinflammation 12/1/2012 [PMID: 22480370]
Määttä M, Tervahartiala T, Kaarniranta K, Tang Y, Yan L, Tuukkanen J, Sorsa T Immunolocalization of EMMPRIN (CD147) in the human eye and detection of soluble form of EMMPRIN in ocular fluids. Curr. Eye Res., 2006-11-01;31(11):917-24. 2006-11-01 [PMID: 17114117] (Western Blot, Human) Western Blot Human
Egawa N, Koshikawa N, Tomari T, Nabeshima K, Isobe T, Seiki M Membrane type 1 matrix metalloproteinase (MT1-MMP/MMP-14) cleaves and releases a 22-kDa extracellular matrix metalloproteinase inducer (EMMPRIN) fragment from tumor cells. J. Biol. Chem., 2006-10-18;281(49):37576-85. 2006-10-18 [PMID: 17050542] (Flow Cytometry, ICC, IHC-P, Human) Flow Cytometry, ICC, IHC-P Human
Haug C, Lenz C, Diaz F, Bachem MG Oxidized low-density lipoproteins stimulate extracellular matrix metalloproteinase Inducer (EMMPRIN) release by coronary smooth muscle cells. Arterioscler. Thromb. Vasc. Biol., 2004-08-19;24(10):1823-9. 2004-08-19 [PMID: 15319264] (ELISA Development, Western Blot, Human) ELISA Development, Western Blot Human

Reviews for EMMPRIN/CD147 Antibody (MAB972) (0)

There are no reviews for EMMPRIN/CD147 Antibody (MAB972). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EMMPRIN/CD147 Antibody (MAB972) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional EMMPRIN/CD147 Products

Blogs on EMMPRIN/CD147.

Check out the latest blog posts on EMMPRIN/CD147.

  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EMMPRIN/CD147 Antibody (109403) [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol BSG
Uniprot