![Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23301_mouse-eea1-mab-m347-19920231856958.jpg "Western Blot: EEA1 Antibody (M347) [NBP3-23301] - Western blot of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-EEA1 antibody at 1:500 (lane 1) or 1:1000 (lane 2).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | M347 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1. [UniProt# Q15075] |
| Specificity | This antibody detects a 180 kDa protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EEA1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 180 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for EEA1 Antibody (NBP3-23301)Find related products by research area.
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Understanding EEA1's Role in Membrane Endosome Fusion EEA1, or Early Endosome Antigen 1 is a Rab5 effector essential for early endocytic membrane fusion. The EEA1 antibody is used in membrane trafficking and chaperone studies, and as an endosome marker. We at Novus Biologicals have a comprehensive antibo... Read full blog post. |
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EEA1 Antibodies in Endosomal Transport and Signalling Studies EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have ... Read full blog post. |
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| Gene Symbol | EEA1 |
![Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] - Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (NBP3-23301). The antibody was detected using goat anti-mouse DyLight(R) 488.](http://images.novusbio.com/fullsize/nbp3-23301_mouse-eea1-mab-m347-19920231856971.jpg "Immunocytochemistry/Immunofluorescence: EEA1 Antibody (M347) [NBP3-23301] - Immunocytochemical labeling of EEA1 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The cells were labeled with mouse monoclonal EEA1 (NBP3-23301). The antibody was detected using goat anti-mouse DyLight(R) 488.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")