![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-344-img0009.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | To produce DYKDDDDK Epitope Tag Antibody, Goats were immunized with ECS, Enterokinase Cleavage Site (xxxDDDDK), conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Specificity | DYKDDDDK Epitope Tag Antibody reacts with FLAG. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in IP, WB, and ICC/IF reported in multiple pieces of scientific literature. |
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| Theoretical MW | 1.01 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DYKDDDDK Epitope Tag Antibody (NB600-344)Find related products by research area.
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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Hoist the DYKDDDDK Epitope Tag The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research... Read full blog post. |
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![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Analysis using the Agarose Immobilized conjugate of DYKDDDDK Epitope Tag Antibody [NB600-344]. DYKDDDK in transfected COS-7 cells using agarose immobilized DYKDDDDK Epitope Tag Antibody.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-344-img0012.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Analysis using the Agarose Immobilized conjugate of DYKDDDDK Epitope Tag Antibody [NB600-344]. DYKDDDK in transfected COS-7 cells using agarose immobilized DYKDDDDK Epitope Tag Antibody.")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI and a PLA against the FLAG epitope (DYKDDDDK Epitope Tag Antibody [NB600-344]) was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122059), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-344-img0013.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI and a PLA against the FLAG epitope (DYKDDDDK Epitope Tag Antibody [NB600-344]) was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122059), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-344-img0011.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).")
![Proximity Ligation Assay: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI & a PLA against the FLAG epitope was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25909512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-344_goat-dykddddk-epitope-tag-pab-bsa-free-31020241535645.jpg "Proximity Ligation Assay: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI & a PLA against the FLAG epitope was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25909512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
