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Cytokeratin 20 Antibody (10D8) [mFluor Violet 610 SE]

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Cytokeratin 20 Antibody (10D8) [mFluor Violet 610 SE] [NBP2-42616MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
10D8
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 610 SE

Order Details

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Cytokeratin 20 Antibody (10D8) [mFluor Violet 610 SE] Summary

Immunogen
Recombinant human Cytokeratin 20 (1-424aa) purified from E.coli.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT20
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cytokeratin 20 Antibody (10D8) [mFluor Violet 610 SE]

  • CD20
  • CK20
  • CK-20
  • cytokeratin-20
  • K20cytokeratin 20
  • keratin 20
  • keratin, type I cytoskeletal 20
  • keratin-20
  • KRT21
  • MGC35423
  • Protein IT

Background

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol KRT20