![Western Blot: CYTIP Antibody [NB100-1002] - Staining of Jurkat (A) and (1 ug/mL) MOLT4 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CYTIP-Antibody-Western-Blot-NB100-1002-img0001.jpg "Western Blot: CYTIP Antibody [NB100-1002] - Staining of Jurkat (A) and (1 ug/mL) MOLT4 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.")
| Reactivity | Hu, Po, CaSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence SLQRLLQHSSNGN-C corresponding to N-Terminus according to NP_004279.3. |
| Predicted Species | Porcine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CYTIP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Approx 38 kDa band observed in lysates of cell line Jurkat and approx 40 kDa in lysates of cell line MOLT4 (calculated MW of 40 kDa according to NP_004279.3). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-1002 | Applications | Species |
|---|---|---|
| Tang P, Cheng TP, Agnello D et al. Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity. Proc Natl Acad Sci U S A 2002-03-05 [PMID: 11867758] |
Secondary Antibodies |
Isotype Controls |
Research Areas for CYTIP Antibody (NB100-1002)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CYTIP |
| Entrez |
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| Uniprot |
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![Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CYTIP-Antibody-Immunofluorescence-NB100-1002-img0002.jpg "Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong cytoplasmic and weak nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CYTIP-Antibody-Immunofluorescence-NB100-1002-img0003.jpg "Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong cytoplasmic and weak nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
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