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Human CXCR4 ELISA Kit (Colorimetric)

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ELISA: Human CXCR4 ELISA Kit (Colorimetric) [NBP3-06841] - Samples were spiked with high concentrations of Human CXCR4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human CXCR4 ELISA Kit (Colorimetric) [NBP3-06841] - Samples were spiked with high concentrations of Human CXCR4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human CXCR4 ELISA Kit (Colorimetric) [NBP3-06841] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

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Human CXCR4 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CXCR4 in samples. No significant cross-reactivity or interference between Human CXCR4 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.67
Intra-Assay
5.14
Spike Recovery
85 - 108 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CXCR4

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human CXCR4 ELISA Kit (Colorimetric)

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201E
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LAP-3
  • LCR1
  • LESTR
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3R
  • NPY3RWHIM
  • NPYR
  • NPYRL
  • NPYY3R
  • pCXCR4
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor
  • WHIMS

Background

CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lympho- and myelopoiesis, cardiogenesis, blood vessel formation and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2. The 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human CXCR4 ELISA
Kit (Colorimetric)
NBP3-06841
Human CXCR4 ELISA Kit (Colorimetric)
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Applications: ELISA

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCR4 ELISA Kit (NBP3-06841). (Showing 1 - 1 of 1 FAQ).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

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Product PDFs

  SDS
  Human CXCR4 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CXCR4