Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.

Name: CXCR4 Antibody
Brand: Novus Biologicals
SKU: NB100-74396
Rating: 3 (1 reviews)

Question

Accepted Answer

CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Immunogen	A synthetic peptide made to a C-terminal region of the human CXCR4 protein (within residues 300-352). [Swiss-Prot P61073]
Localization	Cell membrane; Multi-pass membrane protein. Note: In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	CXCR4
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	Whole antisera
Preservative	0.05% Sodium Azide
Purity	Unpurified

Publications using NB100-74396	Applications	Species
Ranjith Palanisamy, Nimnaka Indrajith Kahingalage, David Archibald, Ilaria Casari, Marco Falasca Synergistic Anticancer Activity of Plumbagin and Xanthohumol Combination on Pancreatic Cancer Models. International journal of molecular sciences 2024-02-26 [PMID: 38397018]
Wang Y, Liu Y, Li XY et al. Vagus nerve stimulation-induced stromal cell-derived factor-l alpha participates in angiogenesis and repair of infarcted hearts ESC heart failure 2023-08-29 [PMID: 37641543] (ICC/IF, Human)	ICC/IF	Human
Zhang S, Peng B, Chen Z et al. Brain-targeting, acid-responsive antioxidant nanoparticles for stroke treatment and drug delivery Bioactive Materials 2022-10-01 [PMID: 35386312]
Tagami M, Kakehashi A, Katsuyama-Yoshikawa A et al. FOXP3 and CXCR4-positive regulatory T cells in the tumor stroma as indicators of tumor immunity in the conjunctival squamous cell carcinoma microenvironment PLOS ONE 2022-03-31 [PMID: 35358193] (IF/IHC, Human)	IF/IHC	Human
Wu, H;Peng, B;Mohammed, FS;Gao, X;Qin, Z;Sheth, KN;Zhou, J;Jiang, Z; Brain Targeting, Antioxidant Polymeric Nanoparticles for Stroke Drug Delivery and Therapy Small (Weinheim an der Bergstrasse, Germany) [PMID: 35306743] (WB, Mouse)	WB	Mouse
Zhang X, Detering L, Sultan D et al. C-X-C Chemokine Receptor Type 4-Targeted Imaging in Glioblastoma Multiforme Using 64Cu-Radiolabeled Ultrasmall Gold Nanoclusters ACS Applied Bio Materials 2021-12-23 [PMID: 35014818] (IHC-Fr)	IHC-Fr
Guan W, Li F, Zhao Z et al. Tumor-Associated Macrophage Promotes the Survival of Cancer Cells upon Docetaxel Chemotherapy via the CSF1/CSF1R-CXCL12/CXCR4 Axis in Castration-Resistant Prostate Cancer Genes 2021-05-19 [PMID: 34069563] (WB, Mouse)	WB	Mouse
Peng C, Chen XT, Xu H et al. Role of the CXCR4/ALK5/Smad3 Signaling Pathway in Cancer-Induced Bone Pain J Pain Res 2020-10-14 [PMID: 33116799] (WB, Rat)	WB	Rat
Zhang, S, Deng, G Et al. Autocatalytic Delivery of Brain Tumor-targeting, Size-shrinkable Nanoparticles for Treatment of Breast Cancer Brain Metastases. Adv Funct Mater 2020-04-03 [PMID: 32440263] (ELISA, Rat)	ELISA	Rat
Zhou Y, Zhang S, Chen Z et al. Targeted Delivery of Secretory Promelittin via Novel Poly(lactone co-beta amino ester) Nanoparticles for Treatment of Breast Cancer Brain Metastases Adv. Sci. 2020-01-19 [PMID: 32154067] (IF/IHC, FLOW, Human)	IF/IHC, FLOW	Human
D'Alterio C, Buoncervello M et al. Targeting CXCR4 potentiates anti-PD-1 efficacy modifying the tumor microenvironment and inhibiting neoplastic PD-1. J Exp Clin Cancer Res 2019-10-28 [PMID: 31661001] (IF/IHC, Mouse)	IF/IHC	Mouse
Ma J, Zhang S, Liu J et al. Targeted Drug Delivery to Stroke via Chemotactic Recruitment of Nanoparticles Coated with Membrane of Engineered Neural Stem Cells Small 2019-07-10 [PMID: 31290245]
Heo GS, Zhao Y, Sultan D, Zhang X et al. Assessment of Copper Nanoclusters for Accurate in Vivo Tumor Imaging and Potential for Translation ACS Appl Mater Interfaces 2019-05-22 [PMID: 31074257] (WB)	WB
Serresi M, Siteur B, Hulsman D et al. Ezh2 inhibition in Kras-driven lung cancer amplifies inflammation and associated vulnerabilities J. Exp. Med. 2018-12-03 [PMID: 30487290] (IHC-P, Human)	IHC-P	Human
Huang X, Wu P, Huang F et al. Baicalin attenuates chronic hypoxia-induced pulmonary hypertension via adenosine A2A receptor-induced SDF-1/CXCR4/PI3K/AKT signaling J. Biomed. Sci. 2017-08-03 [PMID: 28774332] (ICC/IF, Mouse)	ICC/IF	Mouse
Xie C, Gao X, Luo Y et al. Electroacupuncture modulates stromal cell-derived factor-1a expression and mobilization of bone marrow endothelial progenitor cells in focal cerebral ischemia/reperfusion model rats Brain Res. 2016-07-21 [PMID: 27453543] (WB, Human)	WB	Human
Giusti I, Delle Monache S, Di Francesco M et al. From glioblastoma to endothelial cells through extracellular vesicles: messages for angiogenesis. Tumour Biol 2016-09-01 [PMID: 27448307] (Human)		Human
Mayorga M, Kiedrowski M, Shamhart P et al. Early up-regulation of myocardial CXCR4 expression is critical for Dimethyloxalylglycine (DMOG) induced cardiac improvement in acute myocardial infarction (MI). Am. J. Physiol. Heart Circ. Physiol. 2015-10-30 [PMID: 26519029] (IHC-P, Mouse)	IHC-P	Mouse
Lee HW, Cho HJ, Lee SJ et al. Tpl2 induces castration resistant prostate cancer progression and metastasis. Int. J. Cancer. 2014-10-01 [PMID: 25274482] (ICC/IF, WB, IHC-P, Human) Details: CXCR4 antibody used for WB and ICC-IF on PC3 and 22Rv1 cells in relation to Tpl2 (i.e. cells with Tpl2 knockdown and with Tpl2 over-expression) . Antibody also used in IHC-P for the detection of CXCR4 on orthotopic xenograft tumors (Figure 6).	ICC/IF, WB, IHC-P	Human
Gakhar G, Navarro VN, Jurish M et al. Circulating Tumor Cells from Prostate Cancer Patients Interact with E-Selectin under Physiologic Blood Flow. PLoS One 2013-12-27 [PMID: 24386459] (ICC/IF, Human)	ICC/IF	Human
Hoh BL, Hosaka K, Downes DP et al. Stromal cell-derived factor-1 promoted angiogenesis and inflammatory cell infiltration in aneurysm walls. J Neurosurg. 2013-10-25 [PMID: 24160472]
Huang Yf, Chen MJ, Wu MH, Hung SC. The use of hypoxic cultured mesenchymal stem cell for oncolytic virus therapy. Cancer Gene Ther 2013-04-26 [PMID: 23618949]
Mo W, Chen J, Patel A et al. CXCR4/CXCL12 Mediate Autocrine Cell- Cycle Progression in NF1-Associated Malignant Peripheral Nerve Sheath Tumors. Cell 2013-02-28 [PMID: 23434321] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Chen C, Cao J, Song X et al. Adrenaline administration promotes the efficiency of granulocyte colony stimulating factor-mediated hematopoietic stem and progenitor cell mobilization in mice Int J Hematol 2012-12-08 [PMID: 23224606] (WB, Mouse)	WB	Mouse
Kumagai K, Takeuchi R, Ishikawa H et al. Low-intensity pulsed ultrasound accelerates fracture healing by stimulation of recruitment of both local and circulating osteogenic progenitors J Orthop Res 2012-09-01 [PMID: 22419401] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Agarwal U, Ghalayini W, Dong F et al. Role of cardiac myocyte CXCR4 expression in development and left ventricular remodeling after acute myocardial infarction. Circ Res 2010-09-03 [PMID: 20634485] (Mouse)		Mouse
Hasegawa T, McLeod DS, Prow T, Merges C, Grebe R, Lutty GA. Vascular Precursors in Developing Human Retina. Invest Ophthalmol Vis Sci;49(5):2178-92. 2008-05-01 [PMID: 18436851] (IF/IHC, Human)	IF/IHC	Human
Stumm RK, Zhou C, Ara T et al. CXCR4 regulates interneuron migration in the developing neocortex. J Neurosci;23(12):5123-30. 2003-06-15 [PMID: 12832536] (IF/IHC, Rat)	IF/IHC	Rat
Stumm RK, Rummel J, Junker V et al. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci;22(14):5865-78. 2002-07-15 [PMID: 12122049] (WB, IF/IHC, ICC/IF, Rat, Human)	WB, IF/IHC, ICC/IF	Rat, Human
Show All 29 Publications. Collapse Publications.

CXCR4 Antibody

Species Glossary

Images

Product Details

Order Details

CXCR4 Antibody Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for CXCR4 Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for CXCR4 Antibody (NB100-74396)(29)

Review for CXCR4 Antibody (NB100-74396) (1) 31

Summary

Product General Protocols

Video Protocols

FAQs for CXCR4 Antibody (NB100-74396). (Showing 1 - 1 of 1 FAQs).

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional CXCR4 Products

Research Areas for CXCR4 Antibody (NB100-74396)

Blogs on CXCR4.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics

Gene Symbol	CXCR4
Entrez	7852(Human) 12767(Mouse)
Uniprot	P70658()

	Verified Customer 09/11/2014
Application:	WB
Species:	Human