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CXCL7/Thymus Chemokine-1 Antibody (186701)

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Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/Thymus Chemokine‑1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL7/Thymus Chemokine‑1 Monoclonal Antibody (Catalog # MAB1116). The ND50 is typically 4‑20 µg/mL.
Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
186701
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
MAB1116-SP
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CXCL7/Thymus Chemokine-1 Antibody (186701) Summary

Immunogen
E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1
Ile46-Ile107
Accession # NP_714943
Specificity
Detects rat CXCL7/Thymus Chemokine‑1 in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference with recombinant human CXCL5, recombinant mouse CXCL7/Thymus Chemokine-1, recombinant rat (rr) CXCL1/CINC1, rrCXCL2/CINC3, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, or rrCXCL5 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
Ppbp
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 4-20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL7/Thymus Chemokine-1 Antibody (186701)

  • Beta-TG;B-TG1;CTAP3;CTAPIII;CTAP-III;CXCL7;LA-PF4;LDGF;MDGF;NAP-2;PBP;Platelet basic protein;PPBP;SCYB7;TC1;TC2;TGB;TGB1;THBGB;THBGB1
  • Thymus Chemokine1
  • Thymus Chemokine-1

Background

CXCL7, also known as Thymus Chemokine-1, is a member of the alpha (CXC) subfamily of chemokines. It functions as a chemoattractant for neutrophils, macrophages and T cells that express CXCR2/IL-8 R beta . Rat CXCL7 cDNA encodes a 111 amino acid (aa) precursor protein. Rat CXCL7 shares 72% aa sequence identity with mouse CXCL7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol Ppbp
Uniprot