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CXCL7/NAP-2 Antibody (59418) [Unconjugated]

 (6 Publications)
 
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Recombinant Human CXCL7/NAP‑2 (Catalog #393‑NP) induces myeloperox­idase release in the cytochalasin‑B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by Recombinant Human CXCL7/NAP‑2 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL7/NAP‑2 Monoclonal Antibody (Catalog # MAB393). The ND50 is typically 15‑20 µg/mL in the presence of cytochalasin‑B.
Recombinant Human CXCL7/NAP‑2 (Catalog #393‑NP) induces myeloperox­idase release in the cytochalasin‑B treated human neutrophils in a dose-dependent manner (orange line). Myeloperoxidase Release elicited by ...read more
Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 10 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CXCL7/NAP‑2 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N, ELISA(Cap)
Clone
59418
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB393-SP
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CXCL7/NAP-2 Antibody (59418) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CXCL7/NAP-2
Ala59-Asp128
Accession # P02775
Specificity
Detects human CXCL7/NAP‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) CXCL1, 2, 3, 5, 6, 8, 9, 10, or 12 is observed. In Western blots, no cross-reactivity with rhCXCL1, 2, 3, 5, 6, 8, 9, 10, 12, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12 or recombinant rat CXCL1, 2 or 3 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PPBP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 15-20 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human CXCL7/NAP-2 Biotinylated Antibody (Catalog number BAF393)
Standard: Recombinant Human CXCL7/NAP-2 (Catalog number 393-NP)
Publications
Read Publications using
MAB393 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL7/NAP-2 Antibody (59418) [Unconjugated]

  • Beta-TG
  • connective tissue-activating peptide III
  • CTAP III
  • CTAP3CTAP-III
  • CTAPIII
  • CXC chemokine ligand 7
  • C-X-C motif chemokine 7
  • CXCL7
  • CXCL7b-TG1
  • LA-PF4
  • LDGFTC1
  • Leukocyte-derived growth factor
  • low-affinity platelet factor IV
  • Macrophage-derived growth factor
  • MDGFTC2
  • NAP2
  • NAP-2
  • NAP-2-L1
  • neutrophil-activating peptide 2
  • neutrophil-activating peptide-2
  • PBPTHBGB
  • platelet basic protein
  • PPBP
  • pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
  • SCAR10
  • SCYB7beta-thromboglobulin
  • small inducible cytokine B7
  • small inducible cytokine subfamily B, member 7
  • Small-inducible cytokine B7
  • TGB
  • TGB1B-TG1
  • THBGB1
  • thrombocidin 1
  • thrombocidin 2
  • thromboglobulin, beta-1

Background

CXCL7, also known as NAP-2, is a 11 kDa angiogenic alpha -chemokine that is produced by the proteolytic cleavage of the inactive 13 kDa platelet basic protein (PBP). PBP, CXCL7, and C-terminally truncated forms of CXCL7 are secreted by monocytes and platelets. Active CXCL7 binds CXCR2 and induces the chemoattration of monocytes, neutrophils, and endothelial progenitor cells. It also inhibits megakaryocytopoiesis in the bone marrow. Within the region included in this immunogen, human CXCL7 shares 52% aa sequence identity with mouse and rat CXCL7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL7/NAP-2 Antibody (MAB393)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Development, ELISA Development (Capture), Functional Assay, Neutralization.


Filter By Application
ELISA Development
(2)
ELISA Development (Capture)
(1)
Functional Assay
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB393 Applications Species
Mariska Kerstholt, Freek R van de Schoor, Marije Oosting, Simone J C F M Moorlag, Yang Li, Martin Jaeger et al. Identifying platelet-derived factors as amplifiers of B. burgdorferi-induced cytokine production Clinical and Experimental Immunology 10/21/2022 [PMID: 36001729]
K Egan, JP van Geffen, H Ma, B Kevane, A Lennon, S Allen, E Neary, M Parsons, P Maguire, K Wynne, R O' Kennedy, JW Heemskerk, FN Áinle Effect of platelet-derived ?-thromboglobulins on coagulation Thromb. Res., 2017-03-30;154(0):7-15. 2017-03-30 [PMID: 28384443] (Functional Assay, Human) Functional Assay Human
JR Wodicka, AM Chambers, GS Sangha, CJ Goergen, A Panitch Development of a Glycosaminoglycan Derived, Selectin Targeting Anti-Adhesive Coating to Treat Endothelial Cell Dysfunction Pharmaceuticals (Basel), 2017-03-29;10(2):. 2017-03-29 [PMID: 28353658] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
van Bladel ER, Roest M Up-regulation of platelet activation in hemophilia A. Haematologica, 2011-03-21;0(0):. 2011-03-21 [PMID: 21422116] (ELISA Development, Human) ELISA Development Human
Snoep JD, Roest M, Barendrecht AD High platelet reactivity is associated with myocardial infarction in premenopausal women: a population-based case-control study. J. Thromb. Haemost., 2010-01-30;8(5):906-13. 2010-01-30 [PMID: 20128867] (ELISA Development, Human) ELISA Development Human
Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J. Am. Coll. Cardiol., 2006-09-27;48(8):1591-9. 2006-09-27 [PMID: 17045893] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol PPBP
Uniprot