Reactivity | MuSpecies Glossary |
Applications | WB, ELISA(Det) |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Biotin |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant mouse CXCL4/PF4 (R&D Systems, Catalog # 595-P4) Val30-Ser105 Accession # Q9Z126 |
Specificity | Detects mouse CXCL4/PF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) CXCL4, recombinant mouse (rm) MIG, rmI-TAC, rhIL-8, and rhIP-10 is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | PF4 |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | ELISA Capture: Mouse CXCL4/PF4 Antibody (Catalog # MAB595) ELISA Detection: Mouse CXCL4/PF4 Biotinylated Antibody (Catalog # BAF595) Standard: Recombinant Mouse CXCL4/PF4 (Catalog # 595-P4) |
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Readout System | ||
Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
CXCL4 is a member of the CXC chemokine family. Mouse CXCL4 is a 105 amino acid (aa) protein with a 29 aa signal sequence and a 76 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Mouse and human CXCL4 share a 64% identity. Mouse and rat CXCL4 share 89% identity. CXCL4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other fuctions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.
Publication using BAF595 | Applications | Species |
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Brady J, Hayakawa Y, Smyth MJ, Nutt SL IL-21 induces the functional maturation of murine NK cells. J. Immunol., 2004-02-15;172(4):2048-58. 2004-02-15 [PMID: 14764669] (ELISA Development, Mouse) | ELISA Development | Mouse |
Secondary Antibodies |
Isotype Controls |
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