Complement Component C1qA Antibody (JL-1)

The monoclonal antibody JL-1 recognizes the collagen-like region (CLR) of mouse C1q, a 459 kDa molecule consisting of three individual polypeptide chains. The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q. C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway. Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway. C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen. Furthermore, C1q can also recognize molecular patterns associated with pathogens and it can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis. As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto antigens, thereby preventing stimulation of the immune system. C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage. C1q deficiency has a profound effect on host defence and clearance of immune complexes. Absence of C1q may cause autoimmunity by impairment of the clearance of apoptotic cells. Inherited C1q deficiency is also associated with the development of systemic lupus erythematosus (SLE). The monoclonal antibody JL-1 is reactive with the collagen-like region (CLR) only, which is the same region to which autoantibodies in mice and humans are binding. Anti-C1q autoantibodies deposit in glomeruli together with C1q but induce overt renal disease only in the context of glomerular immune complex disease. This provides an explanation why anti-C1q antibodies are especially pathogenic in patients with SLE.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: FLOW, IHC-Fr, WB.

Showing Publications 1 - 2 of 2.

Publications using NBP1-51139	Applications	Species
Li Y, Khan N, Ritzel RM et al. Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brain Journal of Neuroinflammation 2023-08-31 [PMID: 37653491] (FLOW, WB)	FLOW, WB
Bi R, Yin Q, Li H et al. A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury Nature communications 2023-02-14 [PMID: 36788226] (FLOW, IHC-Fr, Mouse)	FLOW, IHC-Fr	Mouse

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Oliver Burton	Flow	Mouse	03/08/2018	View Summary Application Flow Cytometry Sample Tested Peritoneal macrophages Species Mouse Lot 20440M0716-A Comments Comments Anti-C1q was conjugated to PE using a Lightning Link kit for use in flow cytometry. Macrophages are gated on CD45+CD11b+ and T cells are gated on CD45+CD3+ lymphocytes. Dead cells were excluded, and Fc receptors were blocked to prevent non-specific interactions.

Array NBP1-51139

• Complement Component C1qA Antibodies