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Rat Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

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ELISA: Rat Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric) [NBP2-75265] - Samples were spiked with high concentrations of Rat Choline Acetyltransferase/ChAT and diluted with Reference Standard & Sample Diluent ...read more
ELISA: Rat Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric) [NBP2-75265] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Rat Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat ChAT in samples. No significant cross-reactivity or interference between Rat ChAT and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.81%
Intra-Assay
CV% < 4.88%
Spike Recovery
85-102%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CHAT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Choline Acetyltransferase/ChAT ELISA Kit (Colorimetric)

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC 2.3.1.6

Background

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on Choline Acetyltransferase/ChAT.

Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
Choline Acetyltransferase (ChAT) is the enzyme that is responsible for biosynthesis of the neurotransmitter acetylcholine. The majority of acetylcholine is synthesized locally at nerve terminals where ChAT catalyzes the transfer of an acetyl group ...  Read full blog post.

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Bioinformatics

Gene Symbol CHAT