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Human CENPF ELISA Kit (Colorimetric)

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ELISA: Human CENPF ELISA Kit (Colorimetric) [NBP2-75231] - Samples were spiked with high concentrations of Human CENPF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human CENPF ELISA Kit (Colorimetric) [NBP2-75231] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human CENPF ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CENPF in samples. No significant cross-reactivity or interference between Human CENPF and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.05%
Intra-Assay
CV% < 5.85%
Spike Recovery
92-106%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CENPF

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human CENPF ELISA Kit (Colorimetric)

  • AH antigen
  • cell-cycle-dependent 350K nuclear protein
  • CENF
  • CENP-F kinetochore protein
  • CENP-F
  • centromere protein F (350/400kD, mitosin)
  • centromere protein F
  • centromere protein F, 350/400ka (mitosin)
  • centromere protein F, 350/400kDa (mitosin)
  • hcp-1
  • Kinetochore protein CENPF
  • mitosin
  • PRO1779

Background

CENPF (Centromere protein F) is required for proper chromosome segregation in mitosis, and has a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural component localization. Specifically, CENPF is a component of the nuclear matrix during the G2 phase of interphase. In late G2, it associates with the kinetochore and maintains this association through early anaphase. Here, CENPF is required for kinetochore function and appears to be the earliest member to interact with the centromere-kinetochore complex. It then localizes to the spindle midzone in late anaphase and to the intracellular bridge in telophase, where the protein is subsequently degraded. Given this diversity of function, CENPF is expected to play an important role in several mitotic events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Additional CENPF Products

Research Areas for CENPF ELISA Kit (NBP2-75231)

Find related products by research area.

Blogs on CENPF.

CENPF: At the Center-o'-mere Mitotic Division (Infographic)
Centromere protein F (CENPF) also known as Mitosin, AH antigen, and kinetochore protein CENPF, is a protein that associates with the centromere-kinetochore complex. CENPF forms both a homodimer and a heterodimer. CENPF can be found in different cellul...  Read full blog post.

CENPF Antibodies as Potential Cancer Markers
Centromere protein F (CENPF), also named mitosin, is a large human protein of 3113 amino acid residues. Its expression and localization are cell cycle-dependent. The protein levels are low in G1 phase but elevated from S to early M phase. CENPF is a n...  Read full blog post.

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Bioinformatics

Gene Symbol CENPF