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CDK2 [p Tyr15] Antibody (SR1560)

Images

 
Western Blot: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Western blot analysis using NBP3-21552 in (1) HeLa treated with AP; (2) HeLa cell lysate.
Immunohistochemistry: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Immunohistochemical analysis of paraffin-embedded human bladder cancer, using NBP3-21552.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clone
SR1560
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CDK2 [p Tyr15] Antibody (SR1560) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human phospho-CDK2 [Tyr15] (Uniprot #: P24941)
Modification
p Tyr15
Specificity
Detects endogenous levels of phospho-CDK2 [Tyr15]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CDK2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000-1:2000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for CDK2 [p Tyr15] Antibody (SR1560)

  • cdc2-related protein kinase
  • CDK2
  • cell devision kinase 2
  • Cell division protein kinase 2
  • cyclin-dependent kinase 2
  • EC 2.7.11
  • EC 2.7.11.22
  • p33 protein kinase
  • p33(CDK2)

Background

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDK2