![Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-262202413221513.jpg "Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clone | 2C4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human CDK2 [p Tyr15] [UniProt P24941] |
| Modification | p Tyr15 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CDK2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CDK2 Antibody (NBP3-26428)Find related products by research area.
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EZH1 has more to offer than gene repression EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development. Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2. PR... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CDK2 |
![Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate (treated with Pervanadate or not). All lanes: CDK2 [p Tyr15] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.Predicted band size: 34 KDaObserved band size: 34 KDa](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241140949.jpg "Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate (treated with Pervanadate or not). All lanes: CDK2 [p Tyr15] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.Predicted band size: 34 KDaObserved band size: 34 KDa")
![Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Analysis of CDK2 [p Tyr15] Antibody (2C4) diluted at 1:80 and staining in Hela cells (treated with Pervanadate). The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241312220.jpg "Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Analysis of CDK2 [p Tyr15] Antibody (2C4) diluted at 1:80 and staining in Hela cells (treated with Pervanadate). The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
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![Immunocytochemistry RB1 Antibody (607121) [Unconjugated]](https://images.novusbio.com/images2/RB1_MAB6495_Immunocytochemistry_10464.jpg)
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![Immunohistochemistry CIB1 Antibody (791119) [Unconjugated]](https://images.novusbio.com/images2/CIB1_MAB7557_Immunohistochemistry_13917.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")