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CD59 Antibody (YTH53.1) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
YTH53.1
Clonality
Monoclonal
Host
Rat
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65537AF350
Alexa Fluor 405
NB100-65537AF405
Alexa Fluor 488
NB100-65537AF488
Alexa Fluor 532
NB100-65537AF532
Alexa Fluor 594
NB100-65537AF594
Alexa Fluor 647
NB100-65537AF647
Alexa Fluor 700
NB100-65537AF700
Alexa Fluor 750
NB100-65537AF750
Allophycocyanin
NB100-65537APC
Allophycocyanin/Cy7
NB100-65537APCCY7
Biotin
NB100-65537B
DyLight 350
NB100-65537UV
DyLight 405
NB100-65537V
DyLight 488
NB100-65537G
DyLight 550
NB100-65537R
DyLight 594
NB100-65537DL594
DyLight 650
NB100-65537C
DyLight 680
NB100-65537FR
DyLight 755
NB100-65537IR
FITC
NB100-65537F
HRP
NB100-65537H
Janelia Fluor 549
NB100-65537JF549
PE
NB100-65537PE
PE/Atto594
NB100-65537PEATT594
PE/Cy5.5
NB100-65537PECY55
PE/Cy7
NB100-65537PECY7
PerCP
NB100-65537PCP
Unconjugated
NB100-65537
mFluor Violet 450 SE
NB100-65537MFV450
mFluor Violet 500 SE
NB100-65537MFV500
mFluor Violet 610 SE
NB100-65537MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-65537
BSA Free
NB100-65537
BSA Free
NB100-65537-0.025mg
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CD59 Antibody (YTH53.1) [Janelia Fluor® 646] Summary

Additional Information
Clone YTH53.1 was used by HLDA to establish CD designation.
Immunogen
Human Peripheral Blood T cells
Localization
Cell membrane; lipid anchor; GPI anchor.
Specificity
NB100-65537 recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CD59
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD59 Antibody (YTH53.1) [Janelia Fluor® 646]

  • 16.3A5
  • 1F5 antigen
  • 1F5
  • 20 kDa homologous restriction factor
  • CD59 antigen
  • CD59 antigen, complement regulatory protein
  • CD59 glycoprotein
  • CD59 molecule, complement regulatory protein
  • CD59
  • EJ16
  • EJ30
  • EJ30, EL32 and G344)
  • EL32
  • FLJ38134
  • FLJ92039
  • G344
  • HRF20
  • HRF-20
  • human leukocyte antigen MIC11
  • Ly-6-like protein
  • lymphocytic antigen CD59/MEM43
  • MACIF
  • MAC-inhibitory protein
  • MAC-IP
  • MEM43 antigen
  • MEM43
  • membrane attack complex (MAC) inhibition factor
  • Membrane attack complex inhibition factor
  • Membrane inhibitor of reactive lysis
  • MGC2354
  • MIC11
  • MIC11MSK21
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • p18-20
  • Protectin
  • surface anitgen recognized by monoclonal 16.3A5
  • T cell-activating protein

Background

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CD59