![Western Blot: CD59 Antibody (MEM-43/5) [NB500-400] - Fig. 1. Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43).](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Western-Blot-NB500-400-img0001.jpg "Western Blot: CD59 Antibody (MEM-43/5) [NB500-400] - Fig. 1. Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL human peripheral blood T cell leukemia cell line using anti-CD59 (MEM-43/5). Lane 1: original cell lysate Lane 2: material immunoprecipitated with anti-human CD59 (MEM-43).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, IP, CyTOF-ready |
| Clone | MEM-43/5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Additional Information | Clone MEM-43/5 was used by HLDA to establish CD designation. |
| Immunogen | Thymocytes and T lymphocytes. |
| Specificity | The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V; WS Code AS S012 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD59 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western Blot - Non-reducing conditions. This antibody is CyTOF ready. |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD59 Antibody (NB500-400)Find related products by research area.
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![Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43/5) [NB500-400] - Staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Immunohistochemistry-Paraffin-NB500-400-img0002.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43/5) [NB500-400] - Staining of human skin (paraffin sections) using anti-CD59 (MEM-43/5).")
![Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - The PIGAc.1234C>T mutation does not impair terminal hematopoietic differentiation during mesoderm induction. Representative example of FACS analysis of hematopoietic phenotypes in the EB-BLCs from PIGAwt and PIGAc.1234C>T. The zebra plot shows expression of CD59 (X-axis) and CD45 (Y-axis) after three and eight days of hematopoietic differentiation. Unstained PIGAwt cells were used as a control. Abbreviations: CFU-Macrophage (M); CFU-Granulocyte-Macrophage (GM); committed erythroid BFU-E (BFU) and CFU-E (CFU) progenitors; multipotent progenitor cells CFU-GEMM (GEMM). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of anti-CD59 MEM-43/5.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Flow-Cytometry-NB500-400-img0006.jpg "Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - The PIGAc.1234C>T mutation does not impair terminal hematopoietic differentiation during mesoderm induction. Representative example of FACS analysis of hematopoietic phenotypes in the EB-BLCs from PIGAwt and PIGAc.1234C>T. The zebra plot shows expression of CD59 (X-axis) and CD45 (Y-axis) after three and eight days of hematopoietic differentiation. Unstained PIGAwt cells were used as a control. Abbreviations: CFU-Macrophage (M); CFU-Granulocyte-Macrophage (GM); committed erythroid BFU-E (BFU) and CFU-E (CFU) progenitors; multipotent progenitor cells CFU-GEMM (GEMM). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of anti-CD59 MEM-43/5.")
![Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - The PIGAc.1234C>T mutation increases PIGA function compared to PIGAnull hiPSCs.(A). Representative example of FACS analysis CD59 expression in the three hiPSC lines. Overlay histogram shows that CD59 expression was significantly higher in PIGAc.1234 C>T hiPSCs compared to PIGAnull hiPSCs. MFI was 445.4 in PIGAwt hiPSCs and 332.6 in PIGAc.1234C>T hiPSCs (p>0.05, NS). However, MFI in PIGAc.1234C>T hiPSCs was significantly higher than 17.9 in PIGAnull hiPSCs (*pT hiPSCs. PIGAnull hiPSCs (purple), PIGAc.1234C>T hiPSCs (orange) and PIGAwt hiPSCs (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of clone MEM-43/5.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Flow-Cytometry-NB500-400-img0005.jpg "Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - The PIGAc.1234C>T mutation increases PIGA function compared to PIGAnull hiPSCs.(A). Representative example of FACS analysis CD59 expression in the three hiPSC lines. Overlay histogram shows that CD59 expression was significantly higher in PIGAc.1234 C>T hiPSCs compared to PIGAnull hiPSCs. MFI was 445.4 in PIGAwt hiPSCs and 332.6 in PIGAc.1234C>T hiPSCs (p>0.05, NS). However, MFI in PIGAc.1234C>T hiPSCs was significantly higher than 17.9 in PIGAnull hiPSCs (*pT hiPSCs. PIGAnull hiPSCs (purple), PIGAc.1234C>T hiPSCs (orange) and PIGAwt hiPSCs (blue). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of clone MEM-43/5.")
![Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - Generation of PIGAc.1234C>T mutation using the PiggyBac transposon system. Representative FACS analysis CD59 expression in TF1PIGAnull cells transfected with PB-PIGAwt, PB-PIGAc.1234C>T or PB-PIGAtr411. Transfected TF1PIGAnull cells were stained with an APC-conjugated CD59 antibody to assess PIGA gene expression. Non-transfected TF1PIGAnull cells were used as a control. MFI represents mean fluorescence intensity. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of MEM-43/5.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-5-Flow-Cytometry-NB500-400-img0004.jpg "Flow Cytometry: CD59 Antibody (MEM-43/5) [NB500-400] - Generation of PIGAc.1234C>T mutation using the PiggyBac transposon system. Representative FACS analysis CD59 expression in TF1PIGAnull cells transfected with PB-PIGAwt, PB-PIGAc.1234C>T or PB-PIGAtr411. Transfected TF1PIGAnull cells were stained with an APC-conjugated CD59 antibody to assess PIGA gene expression. Non-transfected TF1PIGAnull cells were used as a control. MFI represents mean fluorescence intensity. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0174074), licensed under a CC-BY license. Data from the APC-conjugated form of MEM-43/5.")
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