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CD59 Antibody (BRA-10G) [Janelia Fluor® 585]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, B/N, ICC/IF
Clone
BRA-10G
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-11460AF350
Alexa Fluor 405
NBP3-11460AF405
Alexa Fluor 488
NBP3-11460AF488
Alexa Fluor 532
NBP3-11460AF532
Alexa Fluor 594
NBP3-11460AF594
Alexa Fluor 647
NBP3-11460AF647
Alexa Fluor 700
NBP3-11460AF700
Alexa Fluor 750
NBP3-11460AF750
Allophycocyanin
NBP3-11460APC
Biotin
NBP3-11460B
CoraFluor 1
NBP3-11460CL1
DyLight 350
NBP3-11460UV
DyLight 405
NBP3-11460V
DyLight 488
NBP3-11460G
DyLight 550
NBP3-11460R
DyLight 594
NBP3-11460DL594
DyLight 650
NBP3-11460C
DyLight 680
NBP3-11460FR
DyLight 755
NBP3-11460IR
FITC
NBP3-11460F
HRP
NBP3-11460H
Janelia Fluor 525
NBP3-11460JF525
Janelia Fluor 549
NBP3-11460JF549
Janelia Fluor 635
NBP3-11460JF635
Janelia Fluor 646
NBP3-11460JF646
Janelia Fluor 669
NBP3-11460JF669
PE
NBP3-11460PE
PerCP
NBP3-11460PCP
Unconjugated
NBP2-44699-0.1mg
mFluor Violet 450 SE
NBP3-11460MFV450
mFluor Violet 500 SE
NBP3-11460MFV500
mFluor Violet 610 SE
NBP3-11460MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44699-0.1mg
Azide and BSA Free
NBP3-11460
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CD59 Antibody (BRA-10G) [Janelia Fluor® 585] Summary

Additional Information
Clone BRA-10G was used by HLDA to establish CD designation.
Immunogen
Human K562 tumor cells
Localization
Cell Surface
Specificity
Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This monoclonal antibody is useful for study on GPI-anchored proteins, PNH and CD59 functions.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD59
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD59 Antibody (BRA-10G) [Janelia Fluor® 585]

  • 16.3A5
  • 1F5 antigen
  • 1F5
  • 20 kDa homologous restriction factor
  • CD59 antigen
  • CD59 antigen, complement regulatory protein
  • CD59 glycoprotein
  • CD59 molecule, complement regulatory protein
  • CD59
  • EJ16
  • EJ30
  • EJ30, EL32 and G344)
  • EL32
  • FLJ38134
  • FLJ92039
  • G344
  • HRF20
  • HRF-20
  • human leukocyte antigen MIC11
  • Ly-6-like protein
  • lymphocytic antigen CD59/MEM43
  • MACIF
  • MAC-inhibitory protein
  • MAC-IP
  • MEM43 antigen
  • MEM43
  • membrane attack complex (MAC) inhibition factor
  • Membrane attack complex inhibition factor
  • Membrane inhibitor of reactive lysis
  • MGC2354
  • MIC11
  • MIC11MSK21
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • p18-20
  • Protectin
  • surface anitgen recognized by monoclonal 16.3A5
  • T cell-activating protein

Background

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD59