Home » CD43/Sialophorin » CD43/Sialophorin Antibodies » CD43/Sialophorin Antibody (SPN/839) [CoraFluor™ 1]
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CD43/Sialophorin Antibody (SPN/839) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
SPN/839
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47879AF350
Alexa Fluor 405
NBP2-47879AF405
Alexa Fluor 488
NBP2-47879AF488
Alexa Fluor 532
NBP2-47879AF532
Alexa Fluor 594
NBP2-47879AF594
Alexa Fluor 647
NBP2-47879AF647
Alexa Fluor 700
NBP2-47879AF700
Alexa Fluor 750
NBP2-47879AF750
Allophycocyanin
NBP2-47879APC
Allophycocyanin/Cy7
NBP2-47879APCCY7
Biotin
NBP2-47879B
DyLight 350
NBP2-47879UV
DyLight 405
NBP2-47879V
DyLight 488
NBP2-47879G
DyLight 550
NBP2-47879R
DyLight 594
NBP2-47879DL594
DyLight 650
NBP2-47879C
DyLight 680
NBP2-47879FR
DyLight 755
NBP2-47879IR
FITC
NBP2-47879F
HRP
NBP2-47879H
Janelia Fluor 525
NBP2-47879JF525
Janelia Fluor 549
NBP2-47879JF549
Janelia Fluor 585
NBP2-47879JF585
Janelia Fluor 635
NBP2-47879JF635
Janelia Fluor 646
NBP2-47879JF646
Janelia Fluor 669
NBP2-47879JF669
PE
NBP2-47879PE
PE/Atto594
NBP2-47879PEATT594
PE/Cy5.5
NBP2-47879PECY55
PE/Cy7
NBP2-47879PECY7
PerCP
NBP2-47879PCP
Unconjugated
NBP2-44648-0.1mg
mFluor Violet 450 SE
NBP2-47879MFV450
mFluor Violet 500 SE
NBP2-47879MFV500
mFluor Violet 610 SE
NBP2-47879MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44648-0.1mg
Azide and BSA Free
NBP2-47879-0.1mg
IHC-Prediluted
NBP2-48379
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CD43/Sialophorin Antibody (SPN/839) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant full-length human CD43/Sialophorin protein (Uniprot: P16150)
Localization
Cell surface
Marker
T-Cell Marker
Specificity
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for CD43/Sialophorin Antibody (SPN/839) [CoraFluor™ 1]

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SPN