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CD43/Sialophorin Antibody (SPM503) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM503
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34775AF350
Alexa Fluor 405
NBP2-34775AF405
Alexa Fluor 488
NBP2-34775AF488
Alexa Fluor 532
NBP2-34775AF532
Alexa Fluor 594
NBP2-34775AF594
Alexa Fluor 647
NBP2-34775AF647
Alexa Fluor 700
NBP2-34775AF700
Alexa Fluor 750
NBP2-34775AF750
Allophycocyanin
NBP2-34775APC
Biotin
NBP2-34775B
CoraFluor 1
NBP2-34775CL1
DyLight 350
NBP2-34775UV
DyLight 405
NBP2-34775V
DyLight 488
NBP2-34775G
DyLight 550
NBP2-34775R
DyLight 594
NBP2-34775DL594
DyLight 650
NBP2-34775C
DyLight 680
NBP2-34775FR
DyLight 755
NBP2-34775IR
HRP
NBP2-34775H
Janelia Fluor 525
NBP2-34775JF525
Janelia Fluor 549
NBP2-34775JF549
Janelia Fluor 585
NBP2-34775JF585
Janelia Fluor 635
NBP2-34775JF635
Janelia Fluor 646
NBP2-34775JF646
Janelia Fluor 669
NBP2-34775JF669
PE
NBP2-34775PE
PerCP
NBP2-34775PCP
Unconjugated
NBP2-32822-0.1mg
mFluor Violet 450 SE
NBP2-34775MFV450
mFluor Violet 500 SE
NBP2-34775MFV500
mFluor Violet 610 SE
NBP2-34775MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-32822-0.1mg
IHC-Prediluted
NBP2-44647
Azide and BSA Free
NBP2-34775-0.1mg
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CD43/Sialophorin Antibody (SPM503) [FITC] Summary

Immunogen
Myeloblastic KG1 cells
Localization
Cell surface
Marker
T-Cell Marker
Specificity
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So, a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD43/Sialophorin Antibody (SPM503) [FITC]

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SPN