Any reason for cleaved caspase 3 to be seen as purely nuclear staining or is this just an artifact?

Caspase-3 can translocate from the cytoplasm to the nucleus, usually during apoptosis. We do offer a 100% guarantee that this antibody will be useful as stated on the datasheet also.

The datasheet says that it reacts with the active form of Caspase 3 (17 kDa protein), but every time when I do the WB, I get two bands between 15kDa and 20kDa. The different company's cleaved caspase 3 antibody reacts with 17 and 19 kDa protein, so I wonder which band is specific to yours.

Regarding your question, NB600-1235 has been exclusively validated for IHC, not western blot and it is supposed to detect the active form of caspase3. The inactive Caspase 3 is a 31 kD protein, a heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12). When it is Cleaved by granzyme B, it generates the two active subunits. As a result the two bands you are observing on the gel should be correct. However, we haven't tested this antibody for western blot.

I'd like to get the information about the active/cleaved Caspase-3 (NB600-1235). The datasheet says that it reacts with the active form of Caspase-3 (17 kDa protein), but every time when I do the WB, I get two bands between 15kDa and 20kDa. The different company's cleaved caspase 3 antibody reacts with 17 and 19 kDa protein, so I wonder which band is specific to yours?

According to the data I could find for the active/cleaved caspase 3, the active form should appear at approximately 17kDa and also has a smaller subunit seen at approximately 12kDa. The inactive form is approximately 32kDa. These are the theoretical weights and can vary based on type of gel and samples they are run on. In most cases, it looks like most antibodies detect the active forms at 19 and 17kDa respectively. With this particular product, NB600-1235, we have not yet validated it for use in western blot, so we unfortunately do not have any information for the location of bands.

This is a Rabbit antibody right so Do you think I can use it on rabbit tissue?

This antibody does react to rabbit but if you are using it with an anti-rabbit secondary, you will almost certainly see some background staining. I know that mouse-on-mouse blocking systems exist, but I am not sure if there is a rabbit equivalent at this time. The best way to use NB600-1235 on rabbit tissues would be to have it directly conjugated to biotin, HRP, or whatever detection method you are planning to use. This will eliminate the need for an anti-rabbit secondary and should give you little to no background. We do offer a custom conjugation service, so if you would like to have this antibody conjugated, please send an email to let me know what you would like to have this antibody conjugated to and I can get you a quote.

Caspase-3 Antibody (NB600-1235): Novus Biologicals

Name: Caspase-3 Antibody
Brand: Novus Biologicals
SKU: NB600-1235
Rating: 4 (1 reviews)

- COVID-19 Research Tools
  
  Antibodies
  Primary Antibodies
  Conjugated Antibodies
  
  Sample Size Antibodies
  
  KO Validated Antibodies
  
  Recombinant Antibodies
  
  Antibody Sampler Packs
  
  Antibody Pairs
  
  Secondary Antibodies
  
  Isotype Control Antibodies
  
  Proteins and Peptides
  Peptides & Proteins
  
  Blocking Peptides
  
  Biologically Active Proteins
  
  Lysates and Cell Lines
  Lysates
  
  Stem Cell Lines
- Kits
  Antibody Labeling Kits
  
  ChIP Kits
  
  ELISA Kits
  
  Flow Cytometry Kits
  
  Activators and Inhibitors
  Inhibitors
  
  Activators
  
  Support Products
  Exosome Isolation
  
  Flow Cytometry Beads
  
  IHC Tissue Slides
  
  IHC Microarrays
  
  Western Blot Gels & Buffers
  
  Western Blot Membranes
  
  All Support Products
  
  Bulk and Custom
Products
Our Protocols
- Resources
  Protocols
  
  Video Protocols
  
  Troubleshooting Guides
  
  Posters and Literature
  
  Handbooks and Guides
  
  Calculators
  
  FAQs
  
  Blogs
  
  Diseases
  
  Whitepapers
  
  Antibody Concierge
  
  Webinars
- Tools
  Spectra Viewer
  
  Flow Cytometry Panel Builder
  
  Application
  ELISA
  
  Flow Cytometry
  
  Mass Cytometry (CyTOF)
  
  ICC
  
  IHC
  
  Western Blotting
  
  Interviews
  Faculty
  
  Postdoc
Resources
View Pathways
- By Research Area
  Apoptosis
  
  Autophagy
  
  Cancer
  
  Cell Biology
  
  Cellular Markers
  
  Epigenetics
  
  Epitope Tags
  
  Exosomes Research
  
  Hypoxia
  
  Immunology
  
  Microbiology
  
  Neuroscience
  
  Stem Cells
- By Pathway
  AKT
  
  Apoptosis
  
  Autophagy
  
  ERK1/2/5 Pathway
  
  Hedgehog
  
  Hippo
  
  JNK Pathway
  
  mTOR
  
  Necroptosis
  
  NFKB Signaling
  
  p38 Signaling
  
  TGF Beta
  
  TLR
Research Areas
Get Rewards
- Customer Care
  100% Guarantee
  
  Risk Free Testing
  
  Novus Rewards Program
  
  Publication Rewards Program
  
  New Lab Discount
  
  Scholarship
  
  Travel Grants
  
  Promotions
Customer Care
Novus Careers
- About us
  Careers
  
  Collaborations
  
  Events
  
  Novus Story
  
  Our Team
  
  Philanthropy
  
  Press Releases
  
  Why choose Novus
About Us
- Contact Us
  Customer Service
  
  Distributors
  
  Technical Support
Contact

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, FiSpecies Glossary
Applications	WB, ICC/IF, IHC, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	1.0 mg/ml

Immunogen	This Caspase-3 Antibody was developed against a synthetic peptide corresponding to the cleavage site of human caspase 3 (amino acids 167-175), conjugated to KLH.
Localization	Cytoplasmic and some nuclear.
Specificity	This reacts with the active form of Caspase 3 (17 kDa protein).
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	CASP3
Purity	Affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C. Do not freeze.
Buffer	PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Affinity purified

Publications using NB600-1235	Applications	Species
DENIZ GY, ALTUN S, DENIZ GY Evaluation of nickel-induced brain injuries in rats via oxidative stress and apoptosis: Attenuating effects of hyperoside 2 Conference 2019-01-01
Herrera LR, Johnson RA, McGlynn K et al. The Cancer Testes Antigen, HORMAD1, Limits Genomic Instability in Cancer Cells by Protecting Stalled Replication Forks The Journal of biological chemistry 2023-10-12 [PMID: 37838177] (WB, Human)	WB	Human
Tanyeli - Hesperidin Attenuates Oxidative Ovarian Damage Induced by Ischemia Reperfusion: An Antioxidant, Antiautophagic and Antiapoptotic Agent International Journal of Academic Medicine and Pharmacy Jan 7 2021 (IHC)	IHC
Guler Mc, Tanyeli A, Erdogan Dg Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation. The Iranian Journal of Basic Medical Sciences Jan 1 2021 (IHC)	IHC
Dominika Bębnowska, Rafał Hrynkiewicz, Sylwia Rzeszotek, Marika Freus, Agata Poniewierska-Baran, Paulina Niedźwiedzka-Rystwej, Zhifang Zhang Apoptotic Cell Death in an Animal Model of Virus-Induced Acute Liver Failure—Observations during Lagovirus europaeus /GI.2 Infection International Journal of Molecular Sciences 2024-01-08 [PMID: 38255873]
Kavukcu SB, Ensario?lu HK, Karab?y?k H et al. Cell Death Mechanism of Organometallic Ruthenium(II) and Iridium(III) Arene Complexes on HepG2 and Vero Cells ACS omega 2023-10-10 [PMID: 37841164] (ICC/IF, Human, Primate)	ICC/IF	Human, Primate
Akifumi Yao, Mayuko Nakamura, Hisanori Kohtsuka, Tomoki Sunobe, Toru Miura Gonadal and cellular dynamics during protogynous sex change in the harlequin sandsmelt Parapercis pulchella. Journal of fish biology 2023-12-28 [PMID: 37621220]
Abdelmaksoud H, Osman E, Abdel-Hameed S et al. In vivo evaluation of anticryptosporidial effects of wheat germ extracts in immunocompromised mice Journal of Parasitic Diseases 2022-06-18 [PMID: 36091283]
Abdelmaksoud H, Osman E, Abdel-Hameed S et al. In vivo evaluation of anticryptosporidial effects of wheat germ extracts in immunocompromised mice Journal of Parasitic Diseases Jun 18 2022
Cheng Y, Shi L, Mao H et al. The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets Frontiers in aging neuroscience 2022-06-30 [PMID: 35847680] (IHC)	IHC
Eskiler GG, Turna O, Ozkan AD et al. The response of the canine mammary simple carcinoma and carcinosarcoma cells to 5-aminolaevulinic acid-based photodynamic therapy: An in vitro study Journal of photochemistry and photobiology. B, Biology 2022-07-05 [PMID: 35810598] (WB, Canine)	WB	Canine
Amer SA, El-Araby DA, Tartor H et al. Long-Term Feeding with Curcumin Affects the Growth, Antioxidant Capacity, Immune Status, Tissue Histoarchitecture, Immune Expression of Proinflammatory Cytokines, and Apoptosis Indicators in Nile Tilapia, Oreochromis niloticus Antioxidants (Basel) 2022-05-10 [PMID: 35624801] (IHC)	IHC
Mike JK, Wu KY, White Y et al. Defining Longer-Term Outcomes in an Ovine Model of Moderate Perinatal Hypoxia-Ischemia Developmental Neuroscience 2022-05-19 [PMID: 35588703] (ICC/IF)	ICC/IF
Tanyeli A, Guzel Erdogan D, Comakli S et al. Therapeutic effects of apocynin on ovarian ischemia-reperfusion induced lung injury Biotechnic & histochemistry : official publication of the Biological Stain Commission 2022-02-14 [PMID: 35152781] (IF/IHC)	IF/IHC
Guler Mc, Tanyeli A, Erdogan Dg Urapidil alleviates ovarian torsion detorsion injury via regulating oxidative stress, apoptosis, autophagia, and inflammation. Iran J Basic Med Sci 2021-10-29 [PMID: 34712424]
Iwai H, Ataka K, Suzuki H Et al. Tissue-resident M2 macrophages directly contact primary sensory neurons in the sensory ganglia after nerve injury Journal of neuroinflammation 2021-10-13 [PMID: 34645458] (IF/IHC, Mouse)	IF/IHC	Mouse
Ibrahim Fouad G, Ahmed K The protective impact of Berberine against Doxorubicin-induced nephrotoxicity in Rats Tissue Cell 2021-08-09 [PMID: 34371291]
Iskender H, Yenice G, Terim Kapakin KA et al. Effects of high fructose diet on lipid metabolism and the hepatic NF-kappa B/ SIRT-1 pathway Biotechnic & histochemistry : official publication of the Biological Stain Commission 2021-02-25 [PMID: 33629622]
Goto T, Kuramoto E, Dhar A et al. Neurodegeneration of Trigeminal Mesencephalic Neurons by the Tooth Loss Triggers the Progression of Alzheimer's Disease in 3 Tg-AD Model Mice J. Alzheimers Dis. 2020-07-09 [PMID: 32651317] (IHC-FrFl, IF/IHC, Mouse)	IHC-FrFl, IF/IHC	Mouse
Varmazyari A, Taghizadehghalehjoughi A, Sevim C et al. Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies Toxicol Rep 2020-05-06 [PMID: 32489905] (IF/IHC, Rat)	IF/IHC	Rat
Liu KL, Yu XJ, Sun TZ et al. Effects of seawater immersion on open traumatic brain injury in rabbit model Brain Res. 2020-05-20 [PMID: 32445716] (IF/IHC)	IF/IHC
Wang, F, Wang, Q Et al. Foxo3a aggravates inflammation and induces apoptosis in IL-1-treated rabbit chondrocytes via positively regulating tenascin-c. Folia Histochem Cytobiol 2020-02-01 [PMID: 32003441] (IF/IHC, Drosophila melanogaster)	IF/IHC	Drosophila melanogaster
Deniz GY, Laloglu E, Altun S et al. Antioxidant and anti-apoptotic effects of vitexilactone on cisplatin-induced nephrotoxicity in rats Biotech Histochem 2020-01-21 [PMID: 31961202] (IF/IHC)	IF/IHC
Reynolds JA, Henwood MK, Turtle JD et al. Brain-Dependent Processes Fuel Pain-Induced Hemorrhage After Spinal Cord Injury Front Syst Neurosci 2019-09-10 [PMID: 31551720] (WB, Rat)	WB	Rat
Omur A D, Yildirim B et al. Activity of resveratrol on the influence of aflatoxin B1 on the testes of Sprague dawley rats. Pol J Vet Sci 2019-01-06 [PMID: 31269336] (IF/IHC, Rat)	IF/IHC	Rat
Sevim, Comakli S, Taghizadehghalehjoughi A et al. An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas. Toxicology Reports. 2018-11-01 [PMID: 30560060] (IHC-P, Rat)	IHC-P	Rat
Ozcelik M, Erisir M, Guler O et al. The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats Acta Vet. Brno 2019-01-16 (IHC-P, Rat)	IHC-P	Rat
Tamma R, Ruggieri S, Annese T et al. Bcl6/p53 expression, macrophages/mast cells infiltration and microvascular density in invasive breast carcinoma Oncotarget 2018-04-27 [PMID: 29854311] (IF/IHC, Human)	IF/IHC	Human
Hunziker EB, Lippuner K, Keel MJ et al. A novel organ-slice culturing system to simulate meniscal repair: Proof of concept using a synovium-based pool of meniscoprogenitor cells. J. Orthop. Res. 2016-01-21 [PMID: 26790377] (IHC-P, Human)	IHC-P	Human
Ozkaraca M, Ceribasi S, Ceribasi AO, Kilic A. The Role of Apoptosis and Autophagy in Bovine Abortions Associated with Brucella Spp. Acta Veterinaria-Beograd. 2016-01-01 (IHC-P, Bovine)	IHC-P	Bovine
Turtle JD, Strain MM, ReYnolds JA et al. Pain Input After Spinal Cord Injury (SCI) Undermines Long-Term Recovery and Engages Signal Pathways That Promote Cell Death. Front Syst Neurosci. 2018-06-21 [PMID: 29977195] (WB, Rat)	WB	Rat
Onk D, Onk OA, Erol HS et al. Effect of melatonin on antioxidant capacity, inflammation and apoptotic cell death in lung tissue of diabetic rats Acta Cir Bras 2018-04-01 [PMID: 29768540] (IHC-P, Rat)	IHC-P	Rat
Altun S, ozdemir S, Arslan H. Histopathological effects, responses of oxidative stress, inflammation, apoptosis biomarkers and alteration of gene expressions related to apoptosis, oxidative stress, and reproductive system in chlorpyrifos-exposed common carp (Cyprinus carpio L.) Environ. Pollut. 2017-07-01 [PMID: 28675853] (ICC/IF, Fish)	ICC/IF	Fish
Aksu EH, Kandemir FM, Altun S et al. Ameliorative Effect of Carvacrol on Cisplatin-Induced Reproductive Damage in Male Rats. J. Biochem. Mol. Toxicol. 2016-04-25 [PMID: 27111473] (IHC-P, Rat) Details: Rabbit polyclonal active/cleaved Caspase-3 antibody was used at 1:400 dilution for IHC-P analysis of testes of Sprague-Dawley rats which were subjected to treatments with Cisplatin and/or Carvacrol/CARV, an antioxidant that is naturally found in some pla	IHC-P	Rat
Aksu EH, Ozkaraca M, Kandemir FM et al. Mitigation of paracetamol-induced reproductive damage by chrysin in male rats via reducing oxidative stress Andrologia 2016-02-24 [PMID: 26914515] (IHC-P, Rat)	IHC-P	Rat
de Souza PC, Balasubramanian K, Njoku C et al. OKN-007 decreases tumor necrosis and tumor cell proliferation and increases apoptosis in a preclinical F98 rat glioma model J Magn Reson Imaging 2015-04-29 [PMID: 25920494] (IF/IHC, Rat)	IF/IHC	Rat
Kandemir FM, Ozkaraca M, Yildirim BA et al. Rutin attenuates gentamicin-induced renal damage by reducing oxidative stress, inflammation, apoptosis, and autophagy in rats. Ren Fail 2015-04-01 [PMID: 25613739] (Rat)		Rat
Buyuklu M, Kandemir F, Ozkaraca M et al. Benefical effects of lycopene against contrast medium-induced oxidative stress, inflammation, autophagy, and apoptosis in rat kidney. Hum Exp Toxicol. 2014-09-08 [PMID: 25205736]
Buyuklu M, Mehmet Kandemir F, Ozkaraca M et al. Protective effect of curcumin against contrast induced nephropathy in rat kidney: what is happening to oxidative stress, inflammation, autophagy and apoptosis? Eur Rev Med Pharmacol Sci 2014-03-10 [PMID: 24610611] (IHC-P, Rat)	IHC-P	Rat
Wang L, Wu Y, Tan Y et al. Cytotoxic effects of the quinolone levofloxacin on rabbit meniscus cells. J Appl Toxicol 2013-07-01 [PMID: 23813946] (ICC/IF, Rabbit)	ICC/IF	Rabbit
Jee D, Lee WK. Inhibitory Effect of intravitreal Injection of Bevacizumab on Nerve Growth Factor. Current Eye Research. 2011-10-31 [PMID: 22040304]
Ozcelik M, Erisir M, Guler O et al. The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats Acta Vet. Brno Jan 16 2019 (IHC, Rat)	IHC	Rat
COMAKLI S. GLUFOSINATE-BASED HERBICIDE TOXICITY CAUSE INCREASE CLEAVED CASPASE 3 IN RAT LIVER researchgate.net 1905-07-10 (IF/IHC, Rat)	IF/IHC	Rat
Yaya M, Bird J, Idris B, Amer A. Nitric oxide mediated regulation of retinoic acid induced cartilage breakdown J Histol Histopathol Res 2018-07-10 (IF/IHC, Bovine)	IF/IHC	Bovine
Topcu I, Vatansever S, Bayram E et al. The effects of lornoxicam on neuroprotection following diffuse traumatic brain injury in rats. Turk Neurosurg 2013-01-01 [PMID: 24310460] (IHC-P, Rat)	IHC-P	Rat
Show All 45 Publications. Collapse Publications.

Caspase-3 Antibody

Species Glossary

Images

Product Details

Order Details

Caspase-3 Antibody Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for Caspase-3 Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Caspase-3 Antibody (NB600-1235)(45)

Review for Caspase-3 Antibody (NB600-1235) (1) 41

Summary

Comments

Product General Protocols

Video Protocols

FAQs for Caspase-3 Antibody (NB600-1235). (Showing 1 - 4 of 4 FAQs).

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional Caspase-3 Products

Research Areas for Caspase-3 Antibody (NB600-1235)

Blogs on Caspase-3. Showing 1-10 of 11 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Recent Reviews

Bioinformatics

Jurkat Whole Cell Lysate
Caspase-3 Overexpression Lysate
Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
Jurkat Staurosporine Treated / Untreated Cell Lysate

Gene Symbol	CASP3
Entrez	836(Human) 12367(Mouse) 25402(Rat)
Uniprot	P42574(Rabbit) P42574(Rabbit)

	Verified Customer 02/19/2021
Application:	IHC-Fr
Species:	Mouse