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Caspase-3 Antibody - (active/cleaved)

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Biological Strategies: Immunohistochemistry: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Immunohistochemistry analysis of sectioned tumor tissues from the MDA-MB-231 study. Each section was subjected to ...read more
Biological Strategies: Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - The protein expression levels of Bcl-2, Bax and cleaved caspase-3 were detected in the different groups using Western ...read more
Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Cell apoptosis was detected by flow cytometric analysis, and the percentage of apoptotic cells transfected with miR-200c inhibitor or NC inhibitor and ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Dog ischemic brain stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved)( NB100-56113) at ...read more
Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Irradiated mouse spleen stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved) (NB100-56113) at ...read more
Antidepressants-mediated effect on cellular protein content. HT-22 cells were treated with antidepressants for 48 and 96 h and densitometry analysis of NF-kappa B (b), p16 (c), p21 (d), p27 (e), p53 (f), TRF1 (g), TRF2 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

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Caspase-3 Antibody - (active/cleaved) Summary

Immunogen
This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular) reported in scientific literature (PMID 24804954)
  • Flow Cytometry reported in scientific literature (PMID 28287161)
  • Immunoblotting reported in scientific literature (PMID 27912032)
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 29963272)
  • Immunohistochemistry
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 29864441)
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
Preferentially detects active caspase-3 (large subunit: ~14-21 kDa, and small subunit: ~10 kDa). However, it may also detect pro-caspase-3 (~32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3.
Theoretical MW
31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Staurosporine Treated / Untreated Cell Lysate
Publications
Read Publications using
NB100-56113 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34597692). Rat reactivity reported in scientific literature (PMID:32818590).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-3 Antibody - (active/cleaved)

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-3 Antibody (NB100-56113)(76)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Gerbil.

We have publications tested in 8 applications: FLOW, Flow Cytometry Control, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(1)
Flow Cytometry Control
(1)
ICC/IF
(5)
IF/IHC
(8)
IHC
(3)
IHC-Fr
(1)
IHC-P
(12)
WB
(22)
All Applications
Filter By Species
Human
(13)
Mouse
(24)
Rat
(14)
Gerbil
(1)
All Species
Showing Publications 1 - 10 of 76. Show All 76 Publications.
Publications using NB100-56113 Applications Species
EKEBAS G, ATASEVER A, ALPAY A, YAMAN GRAM D Effect of rosemary extract (Rosmarinus officinalis) on chronic liver injury caused by carbon tetrachloride in rats and determination of hepatic apoptosis Sağlık Bilimleri Dergisi 2019-12-31
Mustafa Ahmed Abdel-Reheim, Merhan E. Ali, Ahmed Gaafar A. Gaafar, Ahmed Amine Ashour Quillaja saponin mitigates methotrexate-provoked renal injury; insight into Nrf-2/Keap-1 pathway modulation with suppression of oxidative stress and inflammation Journal of Pharmaceutical Health Care and Sciences 2024-04-09 [PMID: 38594773]
Chandrakumar S, Santiago Tierno I, Agarwal M et al. Mechanical regulation of retinal vascular inflammation and degeneration in diabetes Diabetes 2023-11-21 [PMID: 37986627] (WB, Mouse) WB Mouse
Wang S, Chang CW, Huang J et al. Gasdermin C sensitizes tumor cells to PARP inhibitor therapy in cancer models The Journal of clinical investigation 2023-10-26 [PMID: 37883181] (WB, Human) WB Human
Abdeljalil SM, Wahdan SA, Elghazaly H, Tolba MF Insights into the therapeutic outcomes of trimetazidine/doxorubicin combination in Ehrlich solid-phase carcinoma mouse tumor model Life sciences 2023-06-21 [PMID: 37352914]
Ruiz TFR, Grigio V, Ferrato LJ et al. Impairment of steroidogenesis and follicle development after bisphenol A exposure during pregnancy and lactation in the ovaries of Mongolian gerbils aged females Molecular and cellular endocrinology 2023-02-20 [PMID: 36813021]
Elkhoely A Liraglutide ameliorates gentamicin-induced acute kidney injury in rats via PGC-1 alpha - mediated mitochondrial biogenesis: Involvement of PKA/CREB and Notch/Hes-1 signaling pathways International immunopharmacology 2022-12-14 [PMID: 36525794] (IF/IHC, Rat) IF/IHC Rat
Albadawy R, Hasanin AH, Agwa SHA et al. Rosavin Ameliorates Hepatic Inflammation and Fibrosis in the NASH Rat Model via Targeting Hepatic Cell Death International Journal of Molecular Sciences 2022-09-05 [PMID: 36077546] (IHC) IHC
Silconi ZB, Rosic V, Benazic S et al. The Pt(S-pr-thiosal)2 and BCL1 Leukemia Lymphoma: Antitumor Activity In Vitro and In Vivo International Journal of Molecular Sciences 2022-07-24 [PMID: 35897737]
Xu H, Ye W, Shi B LncRNA MALAT1 Regulates USP22 Expression Through EZH2-Mediated H3K27me3 Modification to Accentuate Sepsis-Induced Myocardial Dysfunction Cardiovascular toxicology 2022-06-20 [PMID: 35726125]
Show All 76 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-3 Antibody (NB100-56113). (Showing 1 - 1 of 1 FAQs).

  1. I used your NB100-56113 and I was wondering if the positive signal could show up in the cytoplasm
    • The caspase 3 protein is a cytoplasmic protein that can be transported from the cytoplasm to the nucleus during apoptosis, so it is possible that the antibody can be found in both locations.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Caspase-3 Antibody (NB100-56113)

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Blogs on Caspase-3. Showing 1-10 of 11 blog posts - Show all blog posts.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.


  Read full blog post.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Cleaved Caspase-3: A Marker of Programmed Cell Death
What are Caspases?Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1). Environme...  Read full blog post.

Caspase-3, The Executioner of Apoptosis
The Role of Caspase-3 in ApoptosisCaspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinat...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 11 - A proinflammatory caspase that induces the innate immune response
While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

D4-GDI (GDP dissociation inhibitor, RhoGD12)
The D4-GDI protein is a negative regulator of the Ras-related Rho family of small molecule "molecular switch" GTPases. The Rho GTPases modify cell structure and architecture via rapid changes to the actin cytoskeleton and cell membrane. M...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol CASP3