Caspase-3 Antibody - (Pro and Active)

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 3 applications: Chemotaxis, IHC-P, WB.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using NB100-56112	Applications	Species
Seyed Hosseini Fin N, Georgevsky D, Sukkar MB, Golzan SM RAGE and its ligand amyloid beta promote retinal ganglion cell loss following ischemia-reperfusion injury Frontiers in cellular neuroscience 2023-04-12 [PMID: 37124398] (IHC-P, Mouse)	IHC-P	Mouse
Ling X, Jiang X, Li Y et al. Sequential Treatment of Bioresponsive Nanoparticles Elicits Antiangiogenesis and Apoptosis and Synergizes with a CD40 Agonist for Antitumor Immunity ACS nano 2020-12-21 [PMID: 33347262]
Chandrasekaran B, Dahiya N R et al. Chronic exposure to cadmium induces a malignant transformation of benign prostate epithelial cells. Oncogenesis 2020-02-17 [PMID: 32066655] (WB, Human)	WB	Human
Kwon I, Song W, Jang Y et al. Elevation of hepatic autophagy and antioxidative capacity by endurance exercise is associated with suppression of apoptosis in mice Annals of Hepatology [PMID: 31611063] (WB)	WB
Bean GR, Kremer JC, Prudner BC et al. A metabolic synthetic lethal strategy with arginine deprivation and chloroquine leads to cell death in ASS1-deficient sarcomas. Cell Death Dis 2016-10-13 [PMID: 27735949] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Cheng Tc, Lai Cs, Chung Mc et al. Potent anti-cancer effect of 3'-hydroxypterostilbene in human colon xenograft tumors PLoS OnE et al. 2014-11-13 [PMID: 25389774] (WB, Human) Details: Caspase-9 antibody used in WB for the detection of pro and cleaved forms of Caspase 9 protein in lysates of COLO205 cancer cells treated or not with Pterostilbene and 3'-hydroxypterostilbene (Figure 2C)	WB	Human
Samaga KKL, Rao GV, Chandrashekara Reddy G. Synthetic racemates of abyssinone I and II induces apoptosis through mitochondrial pathway in human cervix carcinoma cells. Bioorganic Chemistry. 2014-06-26 [PMID: 25019692] (WB, Human) Details: Fig 6: HeLa	WB	Human
Krajewska Maryla, You Zerong, Rong Juan et al. Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity. PLoS One. 2011-01-01 [PMID: 21957448] (WB, Mouse)	WB	Mouse
Liu Q, Zhao X, Frissora F et al. FTY720 demonstrates promising preclinical activity for chronic lymphocytic leukemia and lymphoblastic leukemia/lymphoma. Blood. 2008-01-01 [PMID: 17761520]
Zhao X, Lapalombella R, Joshi T et al. Targeting CD37-positive lymphoid malignancies with a novel engineered small modular immunopharmaceutical. Blood. 2007-10-01 [PMID: 17440052]
Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004-10-01 [PMID: 15380478] (IHC-P, WB, Canine, Rat, Mouse) Details: Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne	IHC-P, WB	Canine, Rat, Mouse
Knoblach SM, Nikolaeva M, Huang X et al. Multiple caspases are activated after traumatic brain injury: evidence for involvement in functional outcome. J Neurotrauma. 2002-10-01 [PMID: 12427325]
Berger T, Kretzler M. Interaction of DAP3 and FADD only after cellular disruption. Nat Immunol. 2002-01-01 [PMID: 11753396] Details: WB in various cell lines: 1. DAP3 (IMG-295), Fig 1B and 1C.
Svingen PA, Karp JE, Krajewski S et al. Evaluation of Apaf-1 and procaspases-2, -3, -7, -8, and -9 as potential prognostic markers in acute leukemia. Blood. 2000-12-01 [PMID: 11090079]
Krajewski S, Gascoyne RD, Zapata JM et al. Immunolocalization of the ICE/Ced-3-family protease, CPP32 (Caspase-3), in non-Hodgkin's lymphomas, chronic lymphocytic leukemias, and reactive lymph nodes. Blood. 1997-05-15 [PMID: 9160689]
Show All 15 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	01/12/2017	View Summary Application Western Blot Sample Tested BRAIN(Substantia Nigra par compacta) Species Mouse Lot AR14-01040-07

Array NB100-56112

• Caspase-3 Antibodies
• Caspase-3 ELISA Kits
• Caspase-3 Kit
• Caspase-3 Lysates
• Caspase-3 Protein
• Caspase-3 ELISA
• Caspase-3 Proteins and Enzymes