Caspase-3 Antibody (CPP32 4-1-18) - BSA Free

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IF/IHC, IHC-F, IHC-P, WB.

Showing Publications 1 - 10 of 24. Show All 24 Publications. Collapse Publications.

Publications using NB500-210	Applications	Species
Basavaraj P, Hsieh PF, Jiang WP et al. Elucidation of scandenolone as anti-cancer activity through impairment of the metabolic and signaling vulnerabilities in prostate cancer Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-05-29 [PMID: 37257224] (WB, Human)	WB	Human
Liu Z, Wang M, Huang R et al. Novel Indole-Chalcone Derivative-Ligated Platinum(IV) Prodrugs Attenuate Cisplatin Resistance in Lung Cancer through ROS/ER Stress and Mitochondrial Dysfunction Journal of medicinal chemistry 2023-03-22 [PMID: 36946996]
Hsieh P, Jiang W, Basavaraj P Et al. Cell suspension culture extract of Eriobotrya japonica attenuates growth and induces apoptosis in prostate cancer cells via targeting SREBP-1/FASN-driven metabolism and AR Phytomedicine 2021-12-01 [PMID: 34740154] (WB, Human)	WB	Human
Rutledge EA, Parvez RK, Short KM et al. Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease Dev. Biol. 2019-06-23 [PMID: 31242448] (IHC-F, Mouse)	IHC-F	Mouse
Cheng Jiadong, Hu Lan, Yang Zheng et al. 2-Oxonanonoidal Antibiotic Actinolactomycin Inhibits Cancer Progression by Suppressing HIF-1 alpha. Cells 2019-05-10 [PMID: 31083403] (WB, Human)	WB	Human
Martins FF, Aguila MB, Mandarim-de-Lacerda CA. Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-). Acta Histochem. 2017-05-13 [PMID: 28506466]
Graus-Nunes F, Marinho TS, Barbosa-da-Silva S et al. Differential effects of angiotensin receptor blockers on pancreatic islet remodelling and glucose homeostasis in diet-induced obese mice. Mol. Cell. Endocrinol. 2016-10-22 [PMID: 27780713] (IF/IHC, Mouse)	IF/IHC	Mouse
Kim H, Lee KH, Park IA et al. Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer. Virchows Arch. 2015-08-18 [PMID: 26280894] (IHC-P, Human) Details: Caspase 3 antibody was used at 1:300 dilution for IHC-P analysis of tissue sections from human cases of luminal A invasive breast ductal carcinoma. The assay was performed on benchmark automatic immunostaining device and the signal detection was performed using biotinylated anti-mouse secondary antibody -peroxidase-labeled streptavidin - DAB method.	IHC-P	Human
Shibayama Y, Kondo T, Ohya H et al. Upregulation of microRNA-126-5p is associated with drug resistance to cytarabine and poor prognosis in AML patients. Oncol Rep 2015-05-01 [PMID: 25759982]
Hsuan SW, Chyau CC, Hung HY et al. The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer Eur J Nutr 2015-02-27 [PMID: 25720497] (WB, Human)	WB	Human
Wu SH, Chyau CC, Chen JH et al. Anti-cancerous effects of Wasabia japonica extract in Hep3B liver cancer cells via ROS accumulation, DNA damage and p73-mediated apoptosis Journal of Functional Foods 2015-03-02 (WB, Human)	WB	Human
Su C Sann-Joong-Kuey-Jian-Tang decreases the protein expression of mammalian target of rapamycin but increases microtubule associated protein II light chain 3 expression to inhibit human BxPC 3 pancreatic carcinoma cells. Mol Med Rep 2015-01-04 [PMID: 25516264] (WB, Human)	WB	Human
Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014-10-15 [PMID: 25326682] (WB, Human)	WB	Human
Chien SY, Kuo SJ, Chen DR, Su CC. Sann-Joong-Kuey-Jian-Tang decreases the protein expression of Mcl?1 and TCTP and increases that of TNF-alpha and Bax in BxPC?3 pancreatic carcinoma cells. Int J Mol Med 2013-07-01 [PMID: 23652631]
Chen YL, Yan MY, Chien SY et al. Sann-Joong-Kuey-Jian-Tang inhibits hepatocellular carcinoma Hep-G2 cell proliferation by increasing TNF-alpha, Caspase-8, Caspase- 3 and Bax but by decreasing TCTP and Mcl-1 expression in vitro. Mol Med Rep 2013-05-01 [PMID: 23525225] (Human)		Human
Peng Z, Shuangzhu Y, Yongjie J et al. TNF receptor-associated factor 6 regulates proliferation, apoptosis, and invasion of glioma cells. Mol Cell Biochem 2013-01-29 [PMID: 23358926] (WB, Human)	WB	Human
Fuzio P, Ditonno P, Rutigliano M et al. Regulation of TGF-B1 expression by Androgen Deprivation Therapy of prostate cancer Cancer Letters 2012-05-18 [PMID: 22269108] (IF/IHC, Human)	IF/IHC	Human
Hsu L-C, Hsu Y-W, Liang Y-H et al. Induction of Apoptosis in Human Breast Adenocarcinoma Cells MCF-7 by Monapurpyridine A, a New Azaphilone Derivative from Monascus purpureus NTU 568. Molecules. 2012-01-01 [PMID: 22237681]
Dash R, Azab B, Quinn B et al. Apogossypol derivative BI-97C1 (Sabutoclax) targeting Mcl-1 sensitizes prostate cancer cells to mda-7/IL-24-mediated toxicity Proc Natl Acad Sci U S A 2011-05-24 [PMID: 21555592] (WB, Human)	WB	Human
Hsu LC, Hsu YW, Liang YH, Kuo YH, Pan TM. Anti-tumor and anti-inflammatory properties of ankaflavin and monaphilone A from monascus purpureus NTU 568. J Agric Food Chem;59(4):1124-30. 2011-02-23 [PMID: 21261255] (WB, Mouse)	WB	Mouse
Rothberg BEG, Bracken MB, Rimm DL. Tissue Biomarkers for Prognosis in Cutaneous Melanoma: A Systematic Review Meta-analysis. J Natl Cancer Inst;101(7):452-474. 2009-01-01 [PMID: 19318635]
Kulkarni AA, Kingsbury SR, Tudzarova S et al. Cdc7 kinase is a predictor of survival and a novel therapeutic target in epithelial ovarian carcinoma. Clin Cancer Res 2009-04-01 [PMID: 19318489]
Nikiforov MA et al. Tumor cell-selective regulation of NOXA by c-MYC in response to proteasome inhibition. Proc Natl Acad Sci U S A104(49):19488-93. 2007-12-04 [PMID: 18042711]
Rodriguez J, Lazebnik Y. Caspase-9 and APAF-1 form an active holoenzyme. Genes Dev;13(24):3179-84. 1999-12-15 [PMID: 10617566]
Show All 24 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Verified Customer	IHC-Fr	Mouse	12/05/2022	View Summary Application Immunohistochemistry-Frozen Sample Tested Mouse Spleen Species Mouse Lot C7
	3 reviewed by: Verified Customer	WB	Human	08/23/2015	View Summary Application Western Blot Sample Tested Human cancer cell whole cell lysate Species Human
	5 reviewed by: Verified Customer	WB	Human	12/21/2011	View Summary Application Western Blot Sample Tested Human Hepatoblastoma Species Human
	4 reviewed by: Verified Customer	WB	Human	11/21/2011	View Summary Application Western Blot Sample Tested Human Species Human

Array NB500-210

• Caspase-3 Antibodies
• Caspase-3 ELISA Kits
• Caspase-3 Kit
• Caspase-3 Lysates
• Caspase-3 Protein
• Caspase-3 ELISA
• Caspase-3 Proteins and Enzymes