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Human Carbonic Anhydrase IV/CA4 ELISA Kit (Colorimetric)

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ELISA: Human Carbonic Anhydrase IV/CA4 ELISA Kit (Colorimetric) [NBP2-70058] - Samples were spiked with high concentrations of Human Carbonic Anhydrase IV/CA4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Carbonic Anhydrase IV/CA4 ELISA Kit (Colorimetric) [NBP2-70058] - Samples were spiked with high concentrations of Human Carbonic Anhydrase IV/CA4 and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Human Carbonic Anhydrase IV/CA4 ELISA Kit (Colorimetric) [NBP2-70058] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

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Human Carbonic Anhydrase IV/CA4 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CA4 in samples. No significant cross-reactivity or interference between Human CA4 and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.47%
Intra-Assay
CV% < 5.15%
Spike Recovery
91-112%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CA4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Carbonic Anhydrase IV/CA4 ELISA Kit (Colorimetric)

  • CA4
  • CAIV
  • CA-IV
  • Car4
  • Carbonate dehydratase IV
  • carbonic anhydrase 4
  • Carbonic Anhydrase IV
  • carbonic anhydrase IVRP17
  • carbonic dehydratase IV
  • EC 4.2.1.1
  • retinitis pigmentosa 17 (autosomal dominant)
  • RP17

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Human Carbonic Anhydrase IV/CA4 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CA4