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Calmodulin Antibody (SJ16-09)

Images

 
Western Blot: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton ...read more
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (SJ16-09) [NBP2-67413] - MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton ...read more
Immunohistochemistry-Paraffin: Calmodulin Antibody (SJ16-09) [NBP2-67413] - Analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SJ16-09
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Calmodulin Antibody (SJ16-09) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human Calmodulin aa 100-149 / 149. (SwissProt: P0DP23 Human; SwissProt: P0DP24 Human; SwissProt: P0DP25 Human)
Localization
Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CALM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000
Publications
Read Publications using
NBP2-67413 in the following applications:

  • PLA
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Calmodulin Antibody (SJ16-09)

  • CALM
  • CALM1
  • CALM2
  • CALM3
  • CALML2
  • calmodulin 1 (phosphorylase kinase, delta)
  • Calmodulin 1
  • calmodulin
  • CaM
  • CAM1
  • CAM2
  • CAM3
  • CAMB
  • CAMC
  • CAMI
  • CAMIII
  • CPVT4
  • DD132
  • PHKDCAM
  • phosphorylase kinase, delta subunit

Background

Calmoduin (CaM) is a calcium modulator protein and a transducer of calcium signals (1-2). Upon calcium binding, calmodulin undergoes conformational changes and binds and modulates a diverse array of proteins. Calcium-bound CaM (Ca2+-CaM) can assume a variety of shapes depending on the target (3). Ca2+-CaM binds many kinases, phosphatases, signaling proteins, and structural proteins affecting a wide variety of processes including neurotransmitter release, muscle contraction, metabolism, apoptosis, inflammation, membrane protein organization, and cytoskeleton movement (2, 4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calmodulin Antibody (NBP2-67413)(3)

Reviews for Calmodulin Antibody (NBP2-67413) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calmodulin Antibody (NBP2-67413). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an antibody that can bind an engineered calmodulin domain of a fluorescent biosensor. Can you provide me the amino acid sequence that your calmodulin antibodies were raised against so that I can compare them to the sequence I am working with? Can you please provide your sequence identity for this fragment: D Q L T E E Q I A E F K E A F S L F D K D G D G T I T T K E L G T V M R S L G Q N P T E A E L Q D M I N E V D A D G D G T I D F P E F L T M M A R K M W G T D S E E E I R E A F R V F D K D G N G Y I G A A E L R H V M A N L G E R L T D E E V D E M I R V A D I N G D G Q V S Y E E F V Q M M T A K
    • Please see this link to our available calmodulin antibodies. This sequences shares 94% similarity with human calmodulin and does not share 100% similarity with any known calmodulin sequence. Any of our calmodulin antibodies will be predicted to react with this sequence. Any of our polyclonal antibodies will be a good choice and chances are most of the monoclonal antibodies will cross-react as well although, unfortunately, there is no way to predict this with the monoclonals. NBP1-61548 would be my best recommendation for your assay and sequence.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CALM1