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BRD4 Antibody (RAB-C131) [Janelia Fluor® 549] - Chimeric

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, ChIP
Clone
RAB-C131
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20125AF350
Alexa Fluor 405
NBP3-20125AF405
Alexa Fluor 488
NBP3-20125AF488
Alexa Fluor 532
NBP3-20125AF532
Alexa Fluor 594
NBP3-20125AF594
Alexa Fluor 647
NBP3-20125AF647
Alexa Fluor 700
NBP3-20125AF700
Alexa Fluor 750
NBP3-20125AF750
Allophycocyanin
NBP3-20125APC
Biotin
NBP3-20125B
CoraFluor 1
NBP3-20125CL1
DyLight 350
NBP3-20125UV
DyLight 405
NBP3-20125V
DyLight 488
NBP3-20125G
DyLight 550
NBP3-20125R
DyLight 594
NBP3-20125DL594
DyLight 650
NBP3-20125C
DyLight 680
NBP3-20125FR
DyLight 755
NBP3-20125IR
FITC
NBP3-20125F
HRP
NBP3-20125H
Janelia Fluor 525
NBP3-20125JF525
Janelia Fluor 585
NBP3-20125JF585
Janelia Fluor 635
NBP3-20125JF635
Janelia Fluor 646
NBP3-20125JF646
Janelia Fluor 669
NBP3-20125JF669
PE
NBP3-20125PE
PerCP
NBP3-20125PCP
Unconjugated
NBP3-20125-0.2mg
mFluor Violet 450 SE
NBP3-20125MFV450
mFluor Violet 500 SE
NBP3-20125MFV500
mFluor Violet 610 SE
NBP3-20125MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20125-0.2mg
Azide and BSA Free
NBP3-20125-0.2mg
Azide and BSA Free
NBP3-20125-0.025mg
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BRD4 Antibody (RAB-C131) [Janelia Fluor® 549] - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained by recombinant antibody (rAb) phage display recognizing BRD4 protein under non-denaturing conditions.
Epitope
This antibody binds to a folded domain, amino acids 333-460.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
BRD4
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for BRD4 Antibody (RAB-C131) [Janelia Fluor® 549] - Chimeric

  • BRD4
  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Background

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BRD4 Antibody
(RAB-C131) [Janelia F ...
NBP3-20125JF549
BRD4 Antibody (RAB-C131) [Janelia Fluor® 549] - Chimeric
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IP, ChIP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BRD4 Antibody (NBP3-20125JF549) (0)

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Secondary Antibodies

 

Isotype Controls

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Check out the latest blog posts on BRD4.
Epigenetic Control of Autophagy
By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi...  Read full blog post.
Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.
NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.
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Bioinformatics

Gene Symbol BRD4